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Literature summary for 2.4.1.122 extracted from

  • Mendicino, J.; Sivakami, S.; Davila, M.; Chandrasekaran, E.V.
    Purification and properties of UDP-Gal:N-acetylgalactosaminide mucin: beta 1,3-galactosyltransferase from swine trachea mucosa (1982), J. Biol. Chem., 257, 3987-3994.
    View publication on PubMed
Search for more literature for 2.4.1.122

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 activation and stabilization, unstable without Sus scrofa

General Stability

General Stability Organism
stabilization by Triton X-100 or Nonidet P-40 required, unstable without Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00035
-
 asialo-mucin of Cowper's gland
-
 Sus scrofa
0.02
-
 UDP-galactose
-
 Sus scrofa
0.09
-
 asialo-mucin of Cowper's gland calculated on the basis of terminal N-galactosaminyl residues Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Sus scrofa 5783
-
Golgi apparatus major part Sus scrofa 5794
-
membrane inner surface Sus scrofa 16020
-
microsome
-
 Sus scrofa
-
-
additional information subcellular distribution Sus scrofa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
 1 * 82000, SDS-PAGE Sus scrofa
90000
-
 gel filtration in the presence of Triton X-100 Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-galactose + N-acetylgalactosaminide mucin Sus scrofa participates in synthesis of the core portion of O-serine- and O-threonine-linked oligosaccharides in respiratory acidic mucins UDP + beta-D-galactosyl-1,3-N-acetylgalactosaminide mucin
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography on Sepharose 4B containing covalently bound mucin substrate Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
tracheal mucosa
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0194
-
 Golgi membranes Sus scrofa
0.81
-
 purified enzyme Sus scrofa

Storage Stability

Storage Stability Organism
3°C, 0.1% Triton X-100 and 0.1 mg/ml albumin, at least 3 months Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information poor substrates are low-molecular weight acceptors, e.g. N-acetylgalactosamine Sus scrofa ?
-
 ?
additional information no acceptor substrates: type A and type 0 blood group glycoprotein, ovomucoid, ovalbumin, and fetuin devoid of sialic acid and galactose Sus scrofa ?
-
 ?
additional information terminal alpha-2,6-linked sialic acid residues inhibit the enzyme Sus scrofa ?
-
 ?
UDP-galactose + asialo-mucin of Cowper's gland
-
 Sus scrofa UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-asialo-mucin of Cowper's gland
-
 ?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine high specificity for N-acetylgalactosaminyl residues linked to serine or threonine Sus scrofa UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
-
 ?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine most active acceptor: macromolecular mucin glycopeptides with free N-acetylgalactosyl residues linked to polypeptide chain Sus scrofa UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
-
 ?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine glycopeptides with less than 5 amino acids containing terminal N-acetylgalactosaminyl residues Sus scrofa UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
-
 ?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine glycosyl acceptor specificity study Sus scrofa UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
-
 ?
UDP-galactose + N-acetyl-D-galactosamine
-
 Sus scrofa UDP + beta-D-galactosyl-1,3-N-acetyl-D-galactosamine
-
 ?
UDP-galactose + N-acetylgalactosaminide mucin
-
 Sus scrofa UDP + beta-D-galactosyl-1,3-N-acetylgalactosaminide mucin
-
 ?
UDP-galactose + N-acetylgalactosaminide mucin participates in synthesis of the core portion of O-serine- and O-threonine-linked oligosaccharides in respiratory acidic mucins Sus scrofa UDP + beta-D-galactosyl-1,3-N-acetylgalactosaminide mucin
-
 ?
UDP-galactose + N-acetylgalactosaminitol
-
 Sus scrofa UDP + beta-D-galactosyl-(1,3)-N-acetylgalactosaminitol
-
 ?
UDP-galactose + p-nitrophenyl-beta-N-acetylgalactosamide
-
 Sus scrofa UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-p-nitrophenyl
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 82000, SDS-PAGE Sus scrofa

Synonyms

Synonyms Comment Organism
UDP-Gal:N-acetylgalactosaminide mucin:beta1,3-galactosyltransferase
-
 Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
-
 Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.9
-
 Sus scrofa