BRENDA - Enzyme Database show
show all sequences of 2.4.1.12

BcsA and BcsB form the catalytically active core of bacterial cellulose synthase sufficient for in vitro cellulose synthesis

Omadjela, O.; Narahari, A.; Strumillo, J.; Melida, H.; Mazur, O.; Bulone, V.; Zimmer, J.; Proc. Natl. Acad. Sci. USA 110, 17856-17861 (2013)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
cyclic-3',5'-diguanylate
activates cellulose synthesis allosterically and binds BcsA-B with high affinity. The tight association of BcsA's PilZ and GT domains suggests that cyclic-3',5'-diguanylate controls the accessibility of the GT active site. Titrating UDP-Glc at different cyclic-3',5'-diguanylate concentrations shows that the maximum catalytic activity achieved depends on the overall c-di-GMP concentration, whereas the apparent affinity for UDP-Glc remains within 0.1-1.0 mM, comparable with the Km of 0.5 mM for UDP-Glc determined in the presence of 0.030 mM cyclic-3',5'-diguanylate. The cyclic-3',5'-diguanylate binding PilZ domain of cellulose synthase is a part of the catalytic BcsA subunit. Cyclic-3',5'-diguanylate does not alter BcsA's apparent affinity for UDP-Glc, yet it increases BcsA's apparent catalytic rate in vitro at least 10fold
Rhodobacter sphaeroides
Cloned(Commentary)
Commentary
Organism
recombinant expression of subunits BcsA and B in Escherichia coli strain C43
Rhodobacter sphaeroides
General Stability
General Stability
Organism
BcsA-B is catalytically active in a detergent-solubilized state
Rhodobacter sphaeroides
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition by guanosine and adenosine diphosphates
Rhodobacter sphaeroides
UDP
BcsA-B undergoes feedback inhibition by UDP, competitive versus UDP-glucose. This inhibitory effect becomes rate limiting as the concentration of UDP-alpha-D-glucose exceeds that of UDP by about an order of magnitude
Rhodobacter sphaeroides
UDP-alpha-D-xylose
strong inhibition
Rhodobacter sphaeroides
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
monophasic Michaelis-Menten kinetics, kinetic analysis of cellulose synthesis, overview
Rhodobacter sphaeroides
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
inner membrane
associated, the membrane-associated domain of BcsB is required for cellulose synthesis
Rhodobacter sphaeroides
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-glucose + [(1->4)-beta-D-glucosyl]n
Rhodobacter sphaeroides
-
UDP + [(1->4)-beta-D-glucosyl]n+1
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodobacter sphaeroides
Q3J125
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucose + [(1->4)-beta-D-glucosyl]n
-
737161
Rhodobacter sphaeroides
UDP + [(1->4)-beta-D-glucosyl]n+1
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rhodobacter sphaeroides
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
cyclic-3',5'-diguanylate
activates cellulose synthesis allosterically and binds BcsA-B with high affinity. The tight association of BcsA's PilZ and GT domains suggests that cyclic-3',5'-diguanylate controls the accessibility of the GT active site. Titrating UDP-Glc at different cyclic-3',5'-diguanylate concentrations shows that the maximum catalytic activity achieved depends on the overall c-di-GMP concentration, whereas the apparent affinity for UDP-Glc remains within 0.1-1.0 mM, comparable with the Km of 0.5 mM for UDP-Glc determined in the presence of 0.030 mM cyclic-3',5'-diguanylate. The cyclic-3',5'-diguanylate binding PilZ domain of cellulose synthase is a part of the catalytic BcsA subunit. Cyclic-3',5'-diguanylate does not alter BcsA's apparent affinity for UDP-Glc, yet it increases BcsA's apparent catalytic rate in vitro at least 10fold
Rhodobacter sphaeroides
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression of subunits BcsA and B in Escherichia coli strain C43
Rhodobacter sphaeroides
General Stability (protein specific)
General Stability
Organism
BcsA-B is catalytically active in a detergent-solubilized state
Rhodobacter sphaeroides
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition by guanosine and adenosine diphosphates
Rhodobacter sphaeroides
UDP
BcsA-B undergoes feedback inhibition by UDP, competitive versus UDP-glucose. This inhibitory effect becomes rate limiting as the concentration of UDP-alpha-D-glucose exceeds that of UDP by about an order of magnitude
Rhodobacter sphaeroides
UDP-alpha-D-xylose
strong inhibition
Rhodobacter sphaeroides
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
monophasic Michaelis-Menten kinetics, kinetic analysis of cellulose synthesis, overview
Rhodobacter sphaeroides
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
inner membrane
associated, the membrane-associated domain of BcsB is required for cellulose synthesis
Rhodobacter sphaeroides
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-glucose + [(1->4)-beta-D-glucosyl]n
Rhodobacter sphaeroides
-
UDP + [(1->4)-beta-D-glucosyl]n+1
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucose + [(1->4)-beta-D-glucosyl]n
-
737161
Rhodobacter sphaeroides
UDP + [(1->4)-beta-D-glucosyl]n+1
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rhodobacter sphaeroides
General Information
General Information
Commentary
Organism
additional information
BcsA and BcsB form the catalytically active core of bacterial cellulose synthase sufficient for in vitro cellulose synthesis
Rhodobacter sphaeroides
physiological function
in bacteria, cellulose synthesis and translocation is catalyzed by the inner membrane-associated bacterial cellulose synthase (Bcs)A and BcsB subunits. Similar to alginate and poly-beta-1,6 N-acetylglucosamine, bacterial cellulose is implicated in the formation of sessile bacterial communities, termed biofilms, and its synthesis is likewise stimulated by cyclic-di-GMP. The membrane-associated domain of BcsB is required for cellulose synthesis
Rhodobacter sphaeroides
General Information (protein specific)
General Information
Commentary
Organism
additional information
BcsA and BcsB form the catalytically active core of bacterial cellulose synthase sufficient for in vitro cellulose synthesis
Rhodobacter sphaeroides
physiological function
in bacteria, cellulose synthesis and translocation is catalyzed by the inner membrane-associated bacterial cellulose synthase (Bcs)A and BcsB subunits. Similar to alginate and poly-beta-1,6 N-acetylglucosamine, bacterial cellulose is implicated in the formation of sessile bacterial communities, termed biofilms, and its synthesis is likewise stimulated by cyclic-di-GMP. The membrane-associated domain of BcsB is required for cellulose synthesis
Rhodobacter sphaeroides
Other publictions for EC 2.4.1.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736991
Olek
The structure of the catalytic ...
Oryza sativa
Plant Cell
26
2996-3009
2014
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736877
Morgan
Crystallographic snapshot of c ...
Rhodobacter sphaeroides
Nature
493
181-186
2013
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737161
Omadjela
BcsA and BcsB form the catalyt ...
Rhodobacter sphaeroides
Proc. Natl. Acad. Sci. USA
110
17856-17861
2013
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718750
Fugelstad
Functional characterization of ...
Saprolegnia monoica
Biochem. Biophys. Res. Commun.
417
1248-1253
2012
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702850
Lau
-
Molecular cloning of cellulose ...
Shorea parvifolia
Biotechnology
8
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2009
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1
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702925
Yin
The cellulose synthase superfa ...
Arabidopsis thaliana, Oryza sativa, Phaeodactylum tricornutum, Populus trichocarpa, Selaginella moellendorffii, Sorghum bicolor, Vitis vinifera
BMC Plant Biol.
9
99-112
2009
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7
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703730
Timmers
Interactions between membrane- ...
Arabidopsis thaliana
FEBS Lett.
583
978-982
2009
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1
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8
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1
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6
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3
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703825
Fugelstad
Identification of the cellulos ...
Saprolegnia monoica
Fungal Genet. Biol.
46
759-767
2009
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2
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2
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704608
Atanassov
Elucidating the mechanisms of ...
Arabidopsis thaliana
J. Biol. Chem.
284
3833-3841
2009
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705822
Sandhu
Plant cell wall matrix polysac ...
Arabidopsis thaliana, Hordeum vulgare, Oryza sativa
Mol. Plant
2
840-850
2009
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2
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705949
Daras
The thanatos mutation in Arabi ...
Arabidopsis thaliana
New Phytol.
184
114-126
2009
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706179
Wightman
A cellulose synthase-containin ...
Arabidopsis thaliana
Plant Cell Physiol.
50
584-594
2009
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2
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706267
Obembe
Differential expression of cel ...
Solanum tuberosum
Plant Physiol. Biochem.
47
1116-1118
2009
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706876
Kumar
An update on the nomenclature ...
Arabidopsis thaliana, Populus alba, Populus balsamifera, Populus deltoides, Populus euphratica, Populus nigra, Populus simonii, Populus tremula, Populus tremula x Populus alba, Populus tremula x Populus tremuloides, Populus tremuloides, Populus trichocarpa, Populus trichocarpa x Populus deltoides, Populus x canadensis, Populus x tomentiglandulosa
Trends Plant Sci.
14
248-254
2009
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15
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18
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15
15
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685834
Singh
Insight into the early steps o ...
Arabidopsis thaliana
BMC Plant Biol.
8
57
2008
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689478
Grenville-Briggs
Cellulose synthesis in Phytoph ...
Phytophthora infestans
Plant Cell
20
720-738
2008
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689619
Paredez
Genetic evidence that cellulos ...
Arabidopsis thaliana
Plant Physiol.
147
1723-1734
2008
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704868
Lu
Differential expression of thr ...
Eucalyptus grandis
J. Exp. Bot.
59
681-695
2008
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689555
Taylor
Identification of cellulose sy ...
Arabidopsis thaliana
Plant Mol. Biol.
64
161-171
2007
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676809
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661462
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Cellulose synthesis in the Ara ...
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637180
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Modifications of cellulose syn ...
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637181
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Cellulose in cyanobacteria. Or ...
Anabaena sp., Crinalium epipsammum, Desmonostoc muscorum, Gloeocapsa sp., Nostoc punctiforme, Oscillatoria princeps, Oscillatoria sp., Phormidium autumnale, Scytonema hofmannii
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637176
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PROCUSTE1 encodes a cellulose ...
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637175
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Thermal stability of the cellu ...
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Saxena
-
Identification of cellulose sy ...
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Cellulose
4
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1997
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637174
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beta-Glucan synthesis in cotto ...
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Cyclic diguanylic acid stimula ...
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Congo red inhibits in vitro be ...
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Orientation of cell wall beta- ...
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Solubilization of the UDP-gluc ...
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Achievement of high rates of i ...
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Larsen
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beta-(1->4)-D-glucan synthesis ...
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The synthesis of cellulose in ...
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