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Literature summary for 2.4.1.116 extracted from

  • Kamsteeg, J.; van Brederode, J.; van Nigtevecht, G.
    The pH-dependent substrate specificity of UDP-glucose: anthocyandin 3-rhamnosylglucoside, 5-O-glucosyltransferase in petals of Silene dioica: the formation of anthocyanidin 3,5-diglucosides (1980), Z. Pflanzenphysiol., 96, 87-93.
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Activating Compound

Activating Compound Comment Organism Structure
Ca2+ 1 mM CaCl2 stimulates 5-O-glucosylation of cyanidin 3-rhamnosyl-glucoside 1.6fold, pH 7.4 Silene dioica
Co2+ 1 mM activates Silene dioica
Mg2+ 1 mM activates Silene dioica
Mn2+ 1 mM activates Silene dioica
additional information glucosylation of cyanidin 3-glucoside at pH 6.5 is not stimulated by divalent metal ions Silene dioica

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, pH 7.5, inhibits formation of cyanidin 3-rhamnosylglucoside-5-glucoside but has no effect on the formation of cyanidin 3,5-diglucoside at pH 6.5 Silene dioica
Hg2+
-
 Silene dioica
Zn2+
-
 Silene dioica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 UDPglucose
-
 Silene dioica
0.59
-
 UDPglucose reaction with + cyanidin 3-rhamnosylglucoside at pH 6.5 Silene dioica
0.6
-
 UDP-glucose pH 6.5 and pH 7.4 Silene dioica
0.67
-
 UDPglucose reaction with cyanidin-3-glucoside at pH 6.5 Silene dioica
2 3.4 anthocyanidin-3-O-glucoside pH 6.5 Silene dioica
3.6
-
 cyanidin-3-rhamnosylglucoside
-
 Silene dioica
3.6
-
 anthocyanidin-3-O-rutinoside pH 7.4 Silene dioica
23.4
-
 cyanidin-3-glucoside
-
 Silene dioica

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 1 mM, 1.6fold stimulation of glucosyl transfer to anthocyanidin-3-O-rutinoside at pH 7.4, but not of glucosyl transfer to anthocyanidin-3-O-glucoside at pH 6.5 Silene dioica

Organism

Organism UniProt Comment Textmining
Silene dioica
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
petal
-
 Silene dioica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + anthocyanidin 3-O-beta-D-rutinoside at pH 7.4, substrate specificity is pH-dependent Silene dioica UDP + anthocyanidin 3-O-beta-D-rutinoside 5-O-beta-D-glucoside
-
 ?
UDP-glucose + anthocyanidin-3-O-glucoside at pH 6.5, but not at pH 7.4, substrate specificity is pH-dependent Silene dioica UDP + anthocyanidin-3,5-O-diglucoside
-
 ?
UDP-glucose + cyanidin-3-O-D-rhamnosyl-1,6-D-glucoside
-
 Silene dioica UDP + cyanidin-3-O-[D-rhamnosyl-1,6-D-glucoside]-5-O-D-glucoside
-
 r
UDP-glucose + cyanidin-3-O-glucoside
-
 Silene dioica UDP + cyanidin-3,5-diglucoside
-
 ?
UDPglucose + cyanidin 3-O-D-glucoside
-
 Silene dioica UDP + ? depending upon pH, the enzyme catalyzes the formation of either anthocyanidin 3-rhamnosylglucoside or 3,5-diglucoside. Maximal formation of anthocyanidin 3-rhamnosylglucoside-5-gluside at pH 7.4, maximal formation of anthocyanidin 3,5-diglucoside at pH 6.5. Formation of cyanidin 3-rhamnosylglucoside-5-glucoside proceeds 5-10fold faster than the cyanidin 3,5-diglucoside formation ?
UDPglucose + cyanidin 3-O-D-rhamnosylglucoside
-
 Silene dioica UDP + ? depending upon pH, the enzyme catalyzes the formation of either anthocyanidin 3-rhamnosylglucoside or 3,5-diglucoside. Maximal formation of anthocyanidin 3-rhamnosylglucoside-5-gluside at pH 7.4, maximal formation of anthocyanidin 3,5-diglucoside at pH 6.5 ?

Synonyms

Synonyms Comment Organism
UDP-glucose:anthocyanidin 3-rhamnosylglucoside 5-O-glucosyltransferase
-
 Silene dioica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 activity with cyanidin-3-glucoside Silene dioica
6.5
-
 glucosyl transfer to anthocyanidin-3-O-glucoside, substrate specificity is pH-dependent Silene dioica
7.4
-
 activity with cyanidin-3-rhamnosylglucoside Silene dioica
7.4
-
 glucosyl transfer to anthocyanidin 3-O-rutinoside, substrate specificity is pH-dependent Silene dioica

pH Range

pH Minimum pH Maximum Comment Organism
6 7.3 pH 6.0: about 80% of maximal activity, pH 7.3: about 50% of half-maximal activity, reaction with cyanidin 3-glucoside Silene dioica
7 8.5 about half-maximal activity at pH 7 and 8.5, reaction with cyanidin-3-rhamnosylglucoside Silene dioica

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.6
-
 30 min, stable below Silene dioica
8
-
 30 min, about 10% loss of activity Silene dioica
8.5
-
 30 min, about 40% loss of activity Silene dioica