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Literature summary for 2.4.1.115 extracted from

  • Kamsteeg, J.; van Brederode, J.; van Nigtevecht, G.
    Identification and properties of UDP-glucose: cyanidin-3-O-glucosyltransferase isolated from petals of the red campion (Silene dioica) (1978), Biochem. Genet., 16, 1045-1058.
    View publication on PubMed
Search for more literature for 2.4.1.115

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol detergent required for maximal activity Silene dioica
2-Methoxyethanol activation Silene dioica
cetrimide activation Silene dioica
cysteine detergent required for maximal activity Silene dioica
Triton X-100 activation Silene dioica
Tween 20 detergent required for maximal activity Silene dioica
Tween 80 detergent required for maximal activity Silene dioica

General Stability

General Stability Organism
repeated freeze-thawing inactivates Silene dioica

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 2 mM, 18% inhibition Silene dioica
HgCl2 0.1 mM, 85% inhibition Silene dioica
NEM 1.25 mM, 30% inhibition Silene dioica
PCMB 1.25 mM, 93% inhhibition, 2-mercaptoethanol or cysteine restores activity Silene dioica
ZnCl2 2 mM, 22% inhibition Silene dioica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 cyanidin
-
 Silene dioica
0.13
-
 delphinidin
-
 Silene dioica
0.13
-
 pelargonidin
-
 Silene dioica
0.41
-
 UDPglucose
-
 Silene dioica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 1 * 60000, can exist as monomer or dimer, SDS-PAGE Silene dioica
60000
-
 2 * 60000, can exist as monomer or dimer, SDS-PAGE Silene dioica
125000
-
 gel filtration Silene dioica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPglucose + anthocyanidin Silene dioica involved in anthocyanine biosynthesis UDP + anthocyanidin-3-O-glucoside
-
 ?

Organism

Organism UniProt Comment Textmining
Silene dioica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Silene dioica

Source Tissue

Source Tissue Comment Organism Textmining
calyx
-
 Silene dioica
-
leaf young rosette leaves Silene dioica
-
petal highest activity in petals of opening flowers of young plants Silene dioica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0252
-
-
 Silene dioica

Storage Stability

Storage Stability Organism
-17°C, about 40% loss of activity within 3 months Silene dioica
4°C, about 40% loss of activity within 1 week Silene dioica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + delphinidin more slowly than cyanidin Silene dioica UDP + delphinidin 3-O-glucoside
-
 ?
UDP-glucose + pelargonidin more slowly than cyanidin Silene dioica UDP + pelargonidin 3-O-glucoside
-
 ?
UDPglucose + anthocyanidin involved in anthocyanine biosynthesis Silene dioica UDP + anthocyanidin-3-O-glucoside
-
 ?
UDPglucose + cyanidin best substrate Silene dioica UDP + cyanidin 3-O-glucoside
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 60000, can exist as monomer or dimer, SDS-PAGE Silene dioica
monomer 1 * 60000, can exist as monomer or dimer, SDS-PAGE Silene dioica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Silene dioica

pH Range

pH Minimum pH Maximum Comment Organism
6.6 8 50% of maximal activity at pH 6.6 and pH 8.0 Silene dioica