General Stability | Organism |
---|---|
addition of high molarity buffer, 200 mM Tris/HCl, or 2-mercaptoethanol, optimal concentration 42 mM, increases stability considerably | Picea abies |
in dilute solutions addition of 10-20% glycerol or ethylene glycol increases stability | Picea abies |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
coniferin | non-competitive, product inhibition | Picea abies | |
EDTA | no inhibition | Picea abies | |
PCMB | 0.1 mM, complete inhibition | Picea abies | |
UDP | non-competitive, product inhibition | Picea abies |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
UDPglucose | - |
Picea abies | |
0.25 | - |
coniferyl alcohol | - |
Picea abies | |
0.58 | - |
sinapyl alcohol | - |
Picea abies | |
0.64 | - |
Vanillin | - |
Picea abies |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
gel filtration | Picea abies |
50000 | - |
1 * 50000, SDS-PAGE | Picea abies |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDPglucose + coniferyl alcohol | Picea abies | participates in lignification of spruce | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picea abies | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Picea abies |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-glucose + coniferyl alcohol = UDP + coniferin | mechanism | Picea abies |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cambium | - |
Picea abies | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.436 | - |
- |
Picea abies |
Storage Stability | Organism |
---|---|
-20°C, 200 mM Tris/HCl, pH 7.5, 15% glycerol, stable for several months | Picea abies |
0-2°C, several weeks | Picea abies |
glycerol, 15%, stabilizes during storage | Picea abies |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | TDPglucose can replace UDPglucose with 17% efficiency | Picea abies | ? | - |
? | |
UDP-glucose + coniferyl alcohol | forward reaction is about six times faster than the reverse reaction | Picea abies | UDP + coniferin | - |
r | |
UDP-glucose + coniferyl aldehyde | glucosylated at 63% the rate of coniferyl alcohol | Picea abies | UDP + coniferaldehyde-4-O-beta-D-glucopyranoside | - |
? | |
UDP-glucose + sinapaldehyde | glucosylated at 48% the rate of coniferyl alcohol | Picea abies | UDP + sinapaldehyde-4-O-beta-D-glucopyranoside | - |
? | |
UDP-glucose + sinapyl alcohol | glucosylated at 14% of the activity with coniferyl alcohol | Picea abies | UDP + syringin | - |
? | |
UDP-glucose + vanillic acid | glucosylated at 14% of the activity with coniferyl alcohol | Picea abies | UDP + vanillic acid-4-O-beta-D-glucopyranoside | - |
? | |
UDP-glucose + vanillin | - |
Picea abies | UDP + vanillin-4-O-beta-D-glucopyranoside | - |
? | |
UDPglucose + coniferyl alcohol | participates in lignification of spruce | Picea abies | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Picea abies |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
36 | - |
- |
Picea abies |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
Tris-HCl, 0.1-0.2 M, or phosphate buffer | Picea abies |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.2 | pH 5.5: about 50% of maximal activity, pH 8.2: about 70% of maximal activity, phosphate buffer | Picea abies |
6.5 | 8.2 | about half-maximal activity at pH 6.5 and 83% of maximal activity at pH 8.2, Tris buffer | Picea abies |