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Literature summary for 2.4.1.111 extracted from

  • Schmid, G.; Grisebach, H.
    Enzymic synthesis of lignin precursors. Purification and properties of UDP glucose: coniferyl-alcohol glucosyltransferase from cambial sap of spruce (Picea abies L.) (1982), Eur. J. Biochem., 123, 363-370.
    View publication on PubMed

General Stability

General Stability Organism
addition of high molarity buffer, 200 mM Tris/HCl, or 2-mercaptoethanol, optimal concentration 42 mM, increases stability considerably Picea abies
in dilute solutions addition of 10-20% glycerol or ethylene glycol increases stability Picea abies

Inhibitors

Inhibitors Comment Organism Structure
coniferin non-competitive, product inhibition Picea abies
EDTA no inhibition Picea abies
PCMB 0.1 mM, complete inhibition Picea abies
UDP non-competitive, product inhibition Picea abies

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
UDPglucose
-
Picea abies
0.25
-
coniferyl alcohol
-
Picea abies
0.58
-
sinapyl alcohol
-
Picea abies
0.64
-
Vanillin
-
Picea abies

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
gel filtration Picea abies
50000
-
1 * 50000, SDS-PAGE Picea abies

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPglucose + coniferyl alcohol Picea abies participates in lignification of spruce ?
-
?

Organism

Organism UniProt Comment Textmining
Picea abies
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Picea abies

Reaction

Reaction Comment Organism Reaction ID
UDP-glucose + coniferyl alcohol = UDP + coniferin mechanism Picea abies

Source Tissue

Source Tissue Comment Organism Textmining
cambium
-
Picea abies
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.436
-
-
Picea abies

Storage Stability

Storage Stability Organism
-20°C, 200 mM Tris/HCl, pH 7.5, 15% glycerol, stable for several months Picea abies
0-2°C, several weeks Picea abies
glycerol, 15%, stabilizes during storage Picea abies

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information TDPglucose can replace UDPglucose with 17% efficiency Picea abies ?
-
?
UDP-glucose + coniferyl alcohol forward reaction is about six times faster than the reverse reaction Picea abies UDP + coniferin
-
r
UDP-glucose + coniferyl aldehyde glucosylated at 63% the rate of coniferyl alcohol Picea abies UDP + coniferaldehyde-4-O-beta-D-glucopyranoside
-
?
UDP-glucose + sinapaldehyde glucosylated at 48% the rate of coniferyl alcohol Picea abies UDP + sinapaldehyde-4-O-beta-D-glucopyranoside
-
?
UDP-glucose + sinapyl alcohol glucosylated at 14% of the activity with coniferyl alcohol Picea abies UDP + syringin
-
?
UDP-glucose + vanillic acid glucosylated at 14% of the activity with coniferyl alcohol Picea abies UDP + vanillic acid-4-O-beta-D-glucopyranoside
-
?
UDP-glucose + vanillin
-
Picea abies UDP + vanillin-4-O-beta-D-glucopyranoside
-
?
UDPglucose + coniferyl alcohol participates in lignification of spruce Picea abies ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Picea abies

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
36
-
-
Picea abies

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
Tris-HCl, 0.1-0.2 M, or phosphate buffer Picea abies

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.2 pH 5.5: about 50% of maximal activity, pH 8.2: about 70% of maximal activity, phosphate buffer Picea abies
6.5 8.2 about half-maximal activity at pH 6.5 and 83% of maximal activity at pH 8.2, Tris buffer Picea abies