Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glucose 6-phosphate | synthase D, i.e. phosphorylated form, is glucose 6-phosphate dependent, synthase I, i.e. dephosphorylated form, is glucose 6-phosphate independent, interconversion of D and I forms and vice versa | Homo sapiens |
General Stability | Organism |
---|---|
albumin is essential during lyophilization, lyophilized enzyme is stable for years | Homo sapiens |
dithiothreitol stabilizes | Homo sapiens |
glucose 6-phosphate stabilizes | Homo sapiens |
glycogen stabilizes | Homo sapiens |
phosphate stabilizes | Homo sapiens |
spontaneous, temperature-dependent inactivation caused by irreversible conformational changes | Homo sapiens |
sulfate stabilizes | Homo sapiens |
UDP stabilizes | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | high concentrations | Homo sapiens | |
ATP | - |
Homo sapiens | |
dithiothreitol | high concentrations | Homo sapiens | |
ethanol | traces inactivate the enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
390000 | - |
sucrose density gradient centrifugation | Homo sapiens |
410000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + glycogen | Homo sapiens | - |
glycogen + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leukocyte | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21 | - |
synthase I | Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, glycogen-free or glycogen-containing enzyme, lyophilized, more than 3 years, solubilized enzyme several weeks | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + glycogen | - |
Homo sapiens | glycogen + UDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000-85000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
inactivation in a few h | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 9.2 | synthase I, presence of glucose 6-phosphate | Homo sapiens |
7.2 | 8 | synthase I, absence of glucose 6-phosphate | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.6 | - |
Homo sapiens |
7.4 | 8.4 | sensitive to small changes in pH in the presence of Mg2+ | Homo sapiens |