Cloned (Comment) | Organism |
---|---|
recombinant expression of HA-tagged isozyme Pmt1p. In Saccharomyces cerevisiae, C-terminal fusions with HA-, FLAG-, or GFP-epitopes do not affect PMTs mannosyltransferase activity | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | Pmt1p membrane topology | Saccharomyces cerevisiae | 5783 | - |
membrane | - |
Saccharomyces cerevisiae | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P33775 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme partially | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl D-mannosyl phosphate + YATAVK-Btn | photocross-linking reaction based on the peptide YATAVK-Btn that serves as in vitro mannosyl acceptor substrate of Pmt1p/Pmt2p and is O -mannosylated to a similar extent as the known acceptor peptide AcNH-YATAV-CONH2 | Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosyl-YATAVK-Btn | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | - |
Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
dolichyl phosphate-mannose:protein O-mannosyltransferase | - |
Saccharomyces cerevisiae |
PMT | - |
Saccharomyces cerevisiae |
Pmt1p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
evolution | PMT family members are conserved throughout the fungal and animal kingdoms. They are further subdivided into the PMT1, PMT2, and PMT4 subfamilies, which include transferases closely related to Saccharomyces cerevisiae Pmt1p, Pmt2p, and Pmt4p, respectively. In fungi, at least one member of each subfamily is present, whereas in animals only PMT2 and PMT4 subfamily members are conserved. In Saccharomyces, Pmt1p, Pmt2p, and Pmt4p account for the major transferase activities although at least six PMTs (Pmt1p-Pmt6p) are present | Saccharomyces cerevisiae |
physiological function | Dol-P-beta-Man:protein alpha-mannosyltransferases are initiating the essential protein O-mannosylation of secretory and membrane proteins in the endoplasmic reticulum. Pmt1p-loop1 is crucial for cross-linking of the photoreactive peptide | Saccharomyces cerevisiae |