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Literature summary for 2.4.1.109 extracted from

  • Kriangkripipat, T.; Momany, M.
    Aspergillus nidulans protein O-mannosyltransferases play roles in cell wall integrity and developmental patterning (2009), Eukaryot. Cell, 8, 1475-1485.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
PCR amplification, creation of fusion enzymes with replacement cassettes Aspergillus nidulans

Protein Variants

Protein Variants Comment Organism
additional information at 25 and 30°C normal growth of single deletion mutants, reduced growth at high temperatures (37 and 42°C), deltapmtA deletion mutant is more heat sensitive than deltapmtC and deltapmtB, double deletion mutant deltapmtA + deltapmtB is the most heat sensitive, partial restoration of heat tolerance by addition of the osmotic sorbitol, the pmtA deletion mutant is more sensitive to cell wall-perturbing agents Calcofluor and Congo red than the wild-type, at 30°C more germ tubes emerge early compared to wild-type and the other deletion mutants except double deletion mutant deltapmtA + deltapmtB, swollen conidia, partial or no conidiogenous layers, at 42°C 2 to 3times swollen conidia, no germ tubes, fewer conidia Aspergillus nidulans
additional information at 25 and 30°C normal growth of single deletion mutants, reduced growth at high temperatures (37 and 42°C), double deletion mutant deltapmtA + deltapmtB is the most heat sensitive, deltapmtA deletion mutant is more heat sensitive than deltapmtC and deltapmtB, partial restoration of heat tolerance by addition of the osmotic sorbitol or KCl (not conidial production), the deltapmtC deletion mutant is resistant to Calcofluor at all temperatures but sensitive to Congo red at elevated temperatures, at 30°C more multiple germ tubes than in the wild-type and in the other deletion mutants, excessive aerial hyphae, fewer conidiophores on elongated stalks, condigiogenous layers misplaced, at 37°C vesicles and conidiogenous layers swollen, fewer spores at all temperatures at 42°C swollen germ tubes and hyperbranching Aspergillus nidulans
additional information at 25 and 30°C normal growth of single deletion mutants, reduced growth at high temperatures (37 and 42°C), pmtA deletion mutant is more heat sensitive than deltapmtC and deltapmtB, double deletion mutant deltapmtA + deltapmtB is the most heat sensitive, partial restoration of heat tolerance by addition of the osmotic sorbitol or KCl, at 25°C the pmtB deletion mutant grows as well as the wild-type in the presence of cell wall-perturbing agents, at 30 and 37°C it is hypersensitive to Calcofluor, at 42°C it is resistant to Calcofluor, to Congo red it is hypersensitive at 37 and 42°C, resistant at lower temperatures, at 42°C lysis of 30% of hyphen tips, at permissive temperature mostly normal conidiophores, occasional lysed vesicles, at restrictive temperature many swollen conidiophore stalks and lysed vesicles, fewer conidia, at 30°C hyphae are growing from adjacent compartments into lysed areas forming intrahyphal hyphae Aspergillus nidulans
additional information double deletion mutant of pmtA + pmtB is the only viable double mutant, it shows slightly retarded growth even at 25°C and is the most heat sensitive deletion mutant, followed in sensitivity by deltapmtA, deltapmtC, and deltapmtB, partial restoration of heat tolerance by addition of the osmotic sorbitol or KCl (not conidial production), the double deletion mutant is more sensitive to cell wall-perturbing agents Calcofluor and Congo red than the wild-type at all tested temperatures, at 25°C swollen conidiophore stalks, vesicles, and conidiogenous layers, at 30°C more germ tubes emerge early compared to wild-type and the other deletion mutants except deltapmtA, lysis of hyphen tips at permissive temperature, at 37°C vegetative and aerial hyphae badly swollen, conidiophores not detectable, reduced conidia productiondefective polar growth at restrictive temperature, at 30°C hyphae are growing from adjacent compartments into lysed areas forming intrahyphal hyphae Aspergillus nidulans
additional information double deletion mutant of pmtB + pmtC is not viable Aspergillus nidulans
additional information double deletion mutants of pmtA + pmtC is not viable Aspergillus nidulans
additional information the triple deletion mutant of pmtA + pmtB + pmtC is not viable Aspergillus nidulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dolichyl phosphate D-mannose + protein AN5660 Aspergillus nidulans the un-glycosylated 32 kDa protein is an ortholog of Wsc family proteins in Saccharomyces cerevisiae, these proteins serve as sensors of stress, such as high temperature and cell wall-perturbing chemicals dolichyl phosphate + O-D-mannosylprotein AN5660 ?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans Q5B3W9 wild-type strain A850 (gene templates and control), and deletion mutants
-
Aspergillus nidulans Q5BDC1 wild-type strain A850 (gene templates and control), and deletion mutants
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Aspergillus nidulans Q96WN5 wild-type strain A850 (gene templates and control), and deletion mutants
-

Purification (Commentary)

Purification (Comment) Organism
conidia are incubated, filtered and washed with stop buffer (pH 7.0), ground in liquid nitrogen, centrifuged, supernatant centrifuged, pellet with crude membranes resuspended, resolved by SDS-PAGE, immunodetection of target protein AN5660 Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dolichyl phosphate D-mannose + protein AN5660 the un-glycosylated 32 kDa protein is an ortholog of Wsc family proteins in Saccharomyces cerevisiae, these proteins serve as sensors of stress, such as high temperature and cell wall-perturbing chemicals Aspergillus nidulans dolichyl phosphate + O-D-mannosylprotein AN5660 ?
dolichyl phosphate D-mannose + protein AN5660 the non-glycosylated 32 kDa protein is an ortholog of Wsc family proteins in Saccharomyces cerevisiae, these proteins serve as sensors of stress, such as high temperature and cell wall-perturbing chemicals, S-tagged AN5660 is hypo-glycosylated in the deltapmtA and deltapmtC deletion mutants (target for these isoforms), not in the deltapmtB deletion mutant (no target) Aspergillus nidulans dolichyl phosphate + O-D-mannosylprotein AN5660 ?

Synonyms

Synonyms Comment Organism
PmtA Pmt-2 subfamily Aspergillus nidulans
pmtB Pmt-1 subfamily Aspergillus nidulans
pmtC Pmt-4 subfamily Aspergillus nidulans
protein O-mannosyltransferase
-
Aspergillus nidulans

General Information

General Information Comment Organism
malfunction single enzyme deletion mutants are viable, at elevated temperatures they show cell wall-associated defects and increased sensitivity to cell wall-perturbing agents, and defects in developmental patterning, partial restoration of wild-type growth with the osmotic sorbitol Aspergillus nidulans
physiological function role in cell wall integrity pathway and developmental patterning by providing spatial cues Aspergillus nidulans