Literature summary for 2.4.1.10 extracted from

Li, R.; Zhang, T.; Jiang, B.; Mu, W.; Miao, M.
Purification and characterization of an intracellular levansucrase derived from Bacillus methylotrophicus SK 21.002 (2015), Biotechnol. Appl. Biochem., 62, 815-822.

Search for more literature for 2.4.1.10

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	inhibits transfructosylation and hydrolytic activities	Bacillus velezensis
Fe2+	inhibits transfructosylation and hydrolytic activities	Bacillus velezensis
Mn2+	inhibits only the hydrolytic activity	Bacillus velezensis
N-bromosuccinimide	-	Bacillus velezensis
Ni2+	inhibits transfructosylation and hydrolytic activities	Bacillus velezensis
phenylmethanesulfonyl fluoride	-	Bacillus velezensis
Zn2+	inhibits transfructosylation and hydrolytic activities	Bacillus velezensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Michaelis-Menten kinetics	Bacillus velezensis
117.2	-	sucrose	pH 6.5, 40°C	Bacillus velezensis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
intracellular	-	Bacillus velezensis	5622	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activates both transfructosylation and hydrolytic activities	Bacillus velezensis
Mg2+	activates both transfructosylation and hydrolytic activities	Bacillus velezensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	gel filtration	Bacillus velezensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n	Bacillus velezensis	-	D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1	-	?
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n	Bacillus velezensis SK 21.002	-	D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus velezensis	-	-	-
Bacillus velezensis SK 21.002	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native intracellular enzyme 11.8fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration	Bacillus velezensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
135.4	-	purified enzyme, pH 6.5, 40°C	Bacillus velezensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n	-	Bacillus velezensis	D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1	-	?
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n	-	Bacillus velezensis SK 21.002	D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 60000, intracellular enzyme, SDS-PAGE	Bacillus velezensis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	transfructosylation activity	Bacillus velezensis
45	-	hydrolytic activity	Bacillus velezensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	transfructosylation and hydrolytic activity	Bacillus velezensis

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Release 2023.1 (January 2023)