Literature summary for 2.4.1.10 extracted from

Han, W.C.; Byun, S.H.; Kim, M.H.; Sohn, E.H.; Lim, J.D.; Um, B.H.; Kim, C.H.; Kang, S.A.; Jang, K.H.
Production of lactosucrose from sucrose and lactose by a levansucrase from Zymomonas mobilis (2009), J. Microbiol. Biotechnol., 19, 1153-1160.

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Inhibitors

Inhibitors	Comment	Organism	Structure
D-glucose	shows high product inhibition in lactosucrose formation, D-glucose is therefor eliminated from the reaction system of lactosucrose production by glucose oxidase	Zymomonas mobilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sucrose + lactose	Zymomonas mobilis	levansucrase transfers the fructosyl moiety of sucrose to lactose to form lactosucrose	D-glucose + O-beta-D-galactopyranosyl-(1,4)-O-beta-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside	i.e. lactosucrose or 4G-beta-D-galactosylsucrose	?

Organism

Organism	UniProt	Comment	Textmining
Zymomonas mobilis	-	gene levU	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + lactose	levansucrase transfers the fructosyl moiety of sucrose to lactose to form lactosucrose	Zymomonas mobilis	D-glucose + O-beta-D-galactopyranosyl-(1,4)-O-beta-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside	i.e. lactosucrose or 4G-beta-D-galactosylsucrose	?
sucrose + lactose	levansucrase transfers the fructosyl moiety of sucrose to lactose to form lactosucrose, optimization of reaction conditions, 28.5% conversion in absence and 43.2% in presence of glucose oxidase, which eliminates the inhibitory D-glucose reaction product, overview	Zymomonas mobilis	D-glucose + O-beta-D-galactopyranosyl-(1,4)-O-beta-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside	i.e. lactosucrose or 4G-beta-D-galactosylsucrose, product identification and quantification by NMR spectroscopy	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
23	-	-	Zymomonas mobilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Zymomonas mobilis

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
additional information	-	levansucrase is highly unstable at alkaline pH	Zymomonas mobilis

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Release 2023.1 (January 2023)