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Literature summary for 2.4.1.10 extracted from

  • Ortiz-Soto, M.; Rudino-Pinera, E.; Rodriguez-Alegria, M.; Munguia, A.
    Evaluation of cross-linked aggregates from purified Bacillus subtilis levansucrase mutants for transfructosylation reactions (2009), BMC Biotechnol., 9, 68-75.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
additional information covalent immobilization of recombinant purified wild-type and mutant enzymes by glutaraldehyde to give cross-linked enzyme aggregates, CLEAs. Usage of 60% ammonium sulfate, 0.2% glutaraldehyde and 4 mg protein/ml. All CLEAs show higher thermal stability than corresponding soluble enzymes, but in the long term, the operational stability is affected by levan synthesis Bacillus subtilis
R360K the immobilized mutant enzyme shows increased activity and is improved for fructosyl-xyloside synthesis compared to the wild-type enzyme Bacillus subtilis
Y429N the immobilized mutant enzyme shows increased activity and is improved for fructosyl-xyloside synthesis compared to the wild-type enzyme Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 sucrose Bacillus subtilis
-
 D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside
-
 ?
additional information Bacillus subtilis levansucrase catalyzes the synthesis of levan from sucrose, but it may also transfer the fructosyl moiety from sucrose to acceptor molecules included in the reaction medium ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 sucrose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside
-
 ?
additional information levansucrase catalyzes the synthesis of levan from sucrose, but it may also transfer the fructosyl moiety from sucrose to acceptor molecules included in the reaction medium Bacillus subtilis ?
-
 ?
additional information levansucrase catalyzes the synthesis of levan from sucrose, but it may also transfer the fructosyl moiety from sucrose to acceptor molecules. The wild-type enzyme synthesizes levan with molecular weight bimodal distribution, while the mutants R360K and Y429N synthesize only oligosaccharides, substrate specificities, overview Bacillus subtilis ?
-
 ?
sucrose + D-xylose
-
 Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-alpha-D-xylopyranoside NMR product analysis ?