Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is useful in levan production for application in the food and pharmaceutical industry | Zymomonas mobilis |
Cloned (Comment) | Organism |
---|---|
gene levU, overexpression of the His-tagged-LevU and of enzyme fused to a chitin-binding domain in Escherichia coli strain DH5alpha | Zymomonas mobilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enhanced levan production using chitin-binding domain fused levansucrase immobilized on chitin beads | Zymomonas mobilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside | Zymomonas mobilis | - |
D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zymomonas mobilis | - |
strain ATCC 10988, gene levU | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged LevU by metal affinity chromatography, recombinant LevU fused to a chitin-binding domain from Escherichia coli strain DH5alpha by affinity binding and immobilization on chitin beads | Zymomonas mobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside | - |
Zymomonas mobilis | D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 15 | - |
Zymomonas mobilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 30 | inactive above | Zymomonas mobilis |