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Literature summary for 2.4.1.10 extracted from

  • Kang, H.K.; Seo, M.Y.; Seo, E.S.; Kim, D.; Chung, S.Y.; Kimura, A.; Day, D.F.; Robyt, J.F.
    Cloning and expression of levansucrase from Leuconostoc mesenteroides B-512 FMC in Escherichia coli (2005), Biochim. Biophys. Acta, 1727, 5-15.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Leuconostoc mesenteroides

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 1 mM, 86% inhibition Leuconostoc mesenteroides
FeSO4 1 mM, 17% inhibition Leuconostoc mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
26.6
-
sucrose
-
Leuconostoc mesenteroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47100
-
2 * 47100, calculated from sequence Leuconostoc mesenteroides
51700
-
2 * 51700, enzyme including a His-tag, SDS-PAGE Leuconostoc mesenteroides
103000
-
non-denaturing PAGE Leuconostoc mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides Q5IS34
-
-
Leuconostoc mesenteroides B-512 FMC Q5IS34
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
126.6
-
-
Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (2,6-beta-D-fructosyl)n
-
Leuconostoc mesenteroides glucose + (2,6-beta-D-fructosyl)n+1
-
?
sucrose + (2,6-beta-D-fructosyl)n
-
Leuconostoc mesenteroides B-512 FMC glucose + (2,6-beta-D-fructosyl)n+1
-
?

Subunits

Subunits Comment Organism
dimer 2 * 47100, calculated from sequence Leuconostoc mesenteroides
dimer 2 * 51700, enzyme including a His-tag, SDS-PAGE Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
optimal temperature for levan formation Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
levan synthesis Leuconostoc mesenteroides

pH Range

pH Minimum pH Maximum Comment Organism
3.5 8.3 pH 3.5: about 55% of maximal activity, pH 8.3: about 60% of maximal activity, levan synthesis Leuconostoc mesenteroides