Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Leuconostoc mesenteroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 1 mM, 86% inhibition | Leuconostoc mesenteroides | |
FeSO4 | 1 mM, 17% inhibition | Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26.6 | - |
sucrose | - |
Leuconostoc mesenteroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47100 | - |
2 * 47100, calculated from sequence | Leuconostoc mesenteroides |
51700 | - |
2 * 51700, enzyme including a His-tag, SDS-PAGE | Leuconostoc mesenteroides |
103000 | - |
non-denaturing PAGE | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | Q5IS34 | - |
- |
Leuconostoc mesenteroides B-512 FMC | Q5IS34 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Leuconostoc mesenteroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
126.6 | - |
- |
Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,6-beta-D-fructosyl)n | - |
Leuconostoc mesenteroides | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | - |
Leuconostoc mesenteroides B-512 FMC | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 47100, calculated from sequence | Leuconostoc mesenteroides |
dimer | 2 * 51700, enzyme including a His-tag, SDS-PAGE | Leuconostoc mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
optimal temperature for levan formation | Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
levan synthesis | Leuconostoc mesenteroides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8.3 | pH 3.5: about 55% of maximal activity, pH 8.3: about 60% of maximal activity, levan synthesis | Leuconostoc mesenteroides |