Cloned (Comment) | Organism |
---|---|
affinity tagged Lactobacillus reuteri levansucrase produced by Bacillus megaterium | Limosilactobacillus reuteri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Limosilactobacillus reuteri | - |
- |
- |
Limosilactobacillus reuteri 21 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of affinity tagged Lactobacillus reuteri levansucrase produced by Bacillus megaterium. The gentle one-step purification method for the recombinant protein directly from the cell-free growth medium provides an alternative to Escherichia coli production and purification systems, where cell disruption, several centrifugation steps, and often more than one purification step are necessary. The one-step purification yields comparable amounts of pure and active enzyme | Limosilactobacillus reuteri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside | besides the major product, levan, the enzyme produces two galactosyloligofructosides with D-Gal-Fru | Limosilactobacillus reuteri | ? | - |
? | |
alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside | besides the major product, levan, the enzyme produces two galactosyloligofructosides with D-Gal-Fru | Limosilactobacillus reuteri 21 | ? | - |
? | |
alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside | only one mannosyloligofructoside is formed | Limosilactobacillus reuteri | ? | - |
? | |
alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside | only one mannosyloligofructoside is formed | Limosilactobacillus reuteri 21 | ? | - |
? | |
alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside | besides the major product, levan, the enzyme produces at least three different xylosyloligofructosides with D-Xyl-Fru | Limosilactobacillus reuteri | ? | - |
? | |
alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside | besides the major product, levan, the enzyme produces at least three different xylosyloligofructosides with D-Xyl-Fru | Limosilactobacillus reuteri 21 | ? | - |
? |