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Literature summary for 2.4.1.10 extracted from
- Levansucrase of Rahnella aquatilis ATCC33071: gene cloning, expression, and levan formation (1998), Ann. N. Y. Acad. Sci., 864, 506-511.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | enzyme is an important biocatalyst for production of fructose homopolymers, potential usage in bioindustrial fields | Rahnella aquatilis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged enzyme in Escherichia coli DH5alpha | Rahnella aquatilis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid sequence alignment | Zymomonas mobilis |
| additional information | - |
amino acid sequence alignment | Rahnella aquatilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rahnella aquatilis | O54435 | strain ATCC33071, gene lsrA | - |
| Rahnella aquatilis ATCC 33071 | O54435 | strain ATCC33071, gene lsrA | - |
| Zymomonas mobilis | - |
gene levU | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from E. coli as His-tagged protein | Rahnella aquatilis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside | N-terminal catalytic domain, C-terminal glucan-binding domain with glycine-rich repeats | Zymomonas mobilis | |
| sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside | N-terminal catalytic domain, C-terminal glucan-binding domain with glycine-rich repeats | Rahnella aquatilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + (2,6-beta-D-fructosyl)n | - |
Zymomonas mobilis | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? |  |
| sucrose + (2,6-beta-D-fructosyl)n | transfucosylation from sucrose to various acceptors, except sugar alcohols | Rahnella aquatilis | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
| sucrose + (2,6-beta-D-fructosyl)n | the degree of polymerization of levan produced at high temperature is lower than that produced at low temperature | Rahnella aquatilis | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
| sucrose + (2,6-beta-D-fructosyl)n | transfucosylation from sucrose to various acceptors, except sugar alcohols | Rahnella aquatilis ATCC 33071 | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
| sucrose + (2,6-beta-D-fructosyl)n | the degree of polymerization of levan produced at high temperature is lower than that produced at low temperature | Rahnella aquatilis ATCC 33071 | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Zymomonas mobilis |
| additional information | - |
the degree of polymerization of levan produced at high temperature is lower than that produced at low temperature | Rahnella aquatilis |
- |
- |
- |
Zymomonas mobilis |
| 20 | - |
levan production | Rahnella aquatilis |
| 40 | - |
levan formation | Rahnella aquatilis |
| 50 | - |
- |
Zymomonas mobilis |
| 50 | - |
maximal reaction velocity | Rahnella aquatilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | - |
Zymomonas mobilis |
| 4 | 6 | recombinant enzyme | Rahnella aquatilis |
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