Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
31 | - |
phosphate | 80°C, pH 6.5 | Pyrococcus furiosus | |
31 | - |
phosphate | pH 6.8, 80°C | Pyrococcus furiosus | |
70 | - |
maltotriose | 80°C, pH 6.5, direction of glucose 1-phosphate synthesis | Pyrococcus furiosus | |
70 | - |
maltotriose | pH 6.8, 80°C | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
98000 | - |
mass spectrometry | Pyrococcus furiosus |
98000 | - |
x * 98000, SDS-PAGE | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Pyrococcus furiosus | Q8U0Q0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[(1->4)-alpha-D-glucosyl]n + phosphate = [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | sequential bi-bi mechanism | Pyrococcus furiosus | |
[(1->4)-alpha-D-glucosyl]n + phosphate = [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | sequential bi-bi catalytic mechanism | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31 | - |
80°C, pH 6.5, substrate glycogen | Pyrococcus furiosus |
52 | - |
80°C, pH 6.5, substrate maltoheptaose | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycogen + alpha-D-glucose 1-phosphate | 75% of the activity with maltose, 64% of the activity with maltoheptaose | Pyrococcus furiosus | glycogen + phosphate | - |
r | |
glycogen + phosphate | - |
Pyrococcus furiosus | ? + alpha-D-glucose 1-phosphate | - |
r | |
maltoheptaose + alpha-D-glucose 1-phosphate | 80% of the activity with maltose | Pyrococcus furiosus | maltooctaose + phosphate | - |
r | |
maltoheptaose + phosphate | - |
Pyrococcus furiosus | maltohexaose + alpha-D-glucose 1-phosphate | - |
r | |
maltoheptaose + phosphate | 35% of the activity with maltoheptaose | Pyrococcus furiosus | maltohexaose + alpha-D-glucose 1-phosphate | - |
r | |
maltohexaose + alpha-D-glucose 1-phosphate | 82% of the activity with maltose | Pyrococcus furiosus | maltoheptaose + phosphate | - |
r | |
maltohexaose + phosphate | - |
Pyrococcus furiosus | maltopentaose + alpha-D-glucose 1-phosphate | - |
r | |
maltohexaose + phosphate | 85% of the activity with maltoheptaose | Pyrococcus furiosus | maltopentaose + alpha-D-glucose 1-phosphate | - |
r | |
maltopentaose + alpha-D-glucose 1-phosphate | 86% of the activity with maltose | Pyrococcus furiosus | maltohexaose + phosphate | - |
r | |
maltopentaose + phosphate | - |
Pyrococcus furiosus | maltotetraose + alpha-D-glucose 1-phosphate | - |
r | |
maltopentaose + phosphate | 78% of the activity with maltoheptaose | Pyrococcus furiosus | maltotetraose + alpha-D-glucose 1-phosphate | - |
r | |
maltose + alpha-D-glucose 1-phosphate | - |
Pyrococcus furiosus | maltotriose + phosphate | - |
r | |
maltotetraose + alpha-D-glucose 1-phosphate | 90% of the activity with maltose | Pyrococcus furiosus | maltopentaose + phosphate | - |
r | |
maltotetraose + phosphate | - |
Pyrococcus furiosus | maltotriose + alpha-D-glucose 1-phosphate | - |
r | |
maltotetraose + phosphate | 61% of the activity with maltoheptaose | Pyrococcus furiosus | maltotriose + alpha-D-glucose 1-phosphate | - |
r | |
maltotriose + alpha-D-glucose 1-phosphate | 95% of the activity with maltose | Pyrococcus furiosus | maltotetraose + phosphate | - |
r | |
maltotriose + phosphate | - |
Pyrococcus furiosus | maltose + alpha-D-glucose 1-phosphate | - |
r | |
maltotriose + phosphate | 35% of the activity with maltoheptaose | Pyrococcus furiosus | maltose + alpha-D-glucose 1-phosphate | - |
r | |
starch + phosphate | - |
Pyrococcus furiosus | ? + alpha-D-glucose 1-phosphate | - |
r | |
[(1->4)-alpha-D-glucosyl]n + phosphate | broad substrate specificity in both the synthesis and degradation of maltooligosaccharides In the synthetic direction of the enzymatic reaction, the lowest oligosaccharide required for the chain elongation is maltose. In the degradative direction the archaeal enzyme can produce D-glucose-1-phosphate from maltotriose or longer maltooligosaccharides including both glycogen and starch | Pyrococcus furiosus | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 98000, SDS-PAGE | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
alpha-glucan/maltodextrin phosphorylase | - |
Pyrococcus furiosus |
PF1535 | locus name | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
Tris-HCl buffer, 24 h, no loss of activity | Pyrococcus furiosus |
80 | - |
retains its complete activity after 24 h at 80°C in Tris-HCl buffer | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Pyrococcus furiosus |