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Literature summary for 2.4.1.1 extracted from

  • Pinotsis, N.; Leonidas, D.D.; Chrysina, E.D.; Oikonomakos, N.G.; Mavridis, I.M.
    The binding of beta- and gamma-cyclodextrins to glycogen phosphorylase b: kinetic and crystallographic studies (2003), Protein Sci., 12, 1914-1924.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.1

Application

Application Comment Organism
additional information the enzyme is a target for development of inhibitors to prevent unwanted hepatic glycogenolysis under high glucose conditions in treatment of diabetes type 2 in humans Oryctolagus cuniculus

Crystallization (Commentary)

Crystallization (Comment) Organism
native enzyme crystals are soaked in 150 mM gamma-cyclodextrin, or 15 mM beta-cyclodextrin, respectively, and 70 mM maltopentaose or maltoheptaose in fesh solutions of mother liquor containing 10 mM MES, pH 6.7, 0.1 mM EDTA, at least 2 h at room temperature, X-ray diffraction structure determination and analysis of enzyme-inhibitor complexes at 2.3 A and 1.94 A resolution, respectively Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
alpha-cyclodextrin mixed-type competitive inhibition, inhibitor is not bound into the enzyme crystal Oryctolagus cuniculus
beta-cyclodextrin mixed-type competitive inhibition, the inhibitor can be accomodated in the glycogen storage site of T-state enzyme, subsite specificity Oryctolagus cuniculus
gamma-cyclodextrin mixed-type competitive inhibition, the inhibitor can be accomodated in the glycogen storage site of T-state enzyme, subsite specificity Oryctolagus cuniculus
additional information binding and inhibition mechanism, no inhibition by (2,3,6-tri-O-methyl)-gamma-cyclodextrin Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
[(1->4)-alpha-D-glucosyl]n + phosphate = [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate determination and analysis of rgulatory binding sites Oryctolagus cuniculus
a

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Oryctolagus cuniculus
-

Synonyms

Synonyms Comment Organism
glycogen phosphorylase
-
 Oryctolagus cuniculus
GP
-
 Oryctolagus cuniculus
GPb
-
 Oryctolagus cuniculus
phosphorylase b
-
 Oryctolagus cuniculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7.4
-
 gamma-cyclodextrin with respect to glycogen Oryctolagus cuniculus
14.1
-
 beta-cyclodextrin with respect to glycogen Oryctolagus cuniculus
47.1
-
 alpha-cyclodextrin with respect to glycogen Oryctolagus cuniculus