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Literature summary for 2.4.1.1 extracted from
- Purification and characterization of the maize amyloplast stromal 112-kDa starch phosphorylase (2001), Arch. Biochem. Biophys., 388, 155-164.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADPglucose | 2.5 mM, 80% inhibition of stromal phosphorylase | Zea mays |  |
| diethyldicarbonate | 0.84 mM, 50% inhibition | Zea mays | |
| Hg2+ | 1 mM, 77% inhibition of stromal phosphorylase | Zea mays | |
| p-chloromercuriphenylsulfonic acid | 1 mM, 88% inhibition of stromal phosphorylase | Zea mays | |
| Phenylglyoxal | 12 mM, 50% inhibition | Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
amylopectin | stromal phosphorylase, synthesis | Zea mays | |
| 0.028 | - |
amylopectin | stromal phosphorylase, phosphorolysis | Zea mays | |
| 0.08 | - |
maltoheptaose | stromal phosphorylase, synthesis | Zea mays | |
| 0.1 | - |
maltoheptaose | stromal phosphorylase, phosphorolysis | Zea mays | |
| 0.16 | - |
maltohexaose | stromal phosphorylase, synthesis | Zea mays | |
| 0.21 | - |
maltohexaose | stromal phosphorylase, phosphorolysis | Zea mays | |
| 0.23 | - |
maltopentaose | stromal phosphorylase, phosphorolysis | Zea mays | |
| 0.25 | - |
glycogen | stromal phosphorylase, synthesis | Zea mays | |
| 0.27 | - |
maltopentaose | stromal phosphorylase, synthesis | Zea mays | |
| 0.57 | - |
maltotetraose | stromal phosphorylase, synthesis | Zea mays | |
| 0.94 | - |
glycogen | stromal phosphorylase, phosphorolysis | Zea mays | |
| 1.51 | - |
glucose 1-phosphate | stromal phosphorylase | Zea mays | |
| 2.26 | - |
phosphate | stromal phosphorylase | Zea mays | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| amyloplast | stroma | Zea mays | 9501 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 112000 | - |
2 * 112000, stromal phosphorylase, SDS-PAGE | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| stromal phosphorylase, ammonium sulfate, Sephacryl 300 Hr, starch adsorption, Q-Sepharose, Mono Q | Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kernel | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 67.5 | - |
stromal phosphorylase | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + glucose 1-phosphate | - |
Zea mays | amylopectin + phosphate | - |
? | |
| glycogen + glucose 1-phosphate | - |
Zea mays | glycogen + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 112000, stromal phosphorylase, SDS-PAGE | Zea mays |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Zea mays |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stromal phosphorylase, 10% losss of activity after 15 min | Zea mays |
| 60 | - |
stromal phosphorylase, 93% loss of activity after 15 min | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7 | phosphorolysis | Zea mays |
| 5.5 | 6.5 | synthesis | Zea mays |
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