Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5'-AMP | 1 mM, 200fold activation of phosphorylase b, completely abolished by caffeine, half-maximal activation of phosphorylases b, ab and a at 0.466, 0.0154 and 0.001 mM AMP respectively | Manduca sexta |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Manduca sexta | |
Caffeine | 5 mM, complete inhibition of phosphorylase b, strong inhibition of phosphorylases ab and a | Manduca sexta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
glycogen | phosphorylase a | Manduca sexta | |
0.55 | - |
glycogen | phosphorylase ab | Manduca sexta | |
2.01 | - |
phosphate | phosphorylase a | Manduca sexta | |
3.03 | - |
phosphate | phosphorylase ab | Manduca sexta | |
6.04 | - |
glycogen | phosphorylase b | Manduca sexta | |
17.89 | - |
phosphate | phosphorylase b | Manduca sexta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
92000 | - |
2 * 92000, phosphorylase a, ab and b, SDS-PAGE | Manduca sexta |
178000 | - |
phosphorylases a, ab and b, native PAGE | Manduca sexta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Manduca sexta | - |
hawk moth | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | in vitro phosphorylation of phosphorylase b with phosphorylase kinase from rabbit muscle | Manduca sexta |
Purification (Comment) | Organism |
---|---|
phosphorylases b, ab and a, DEAE-Sephacel, AMP-Sepharose | Manduca sexta |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | flight muscle | Manduca sexta | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.11 | - |
phosphorylase b | Manduca sexta |
10.08 | - |
phosphorylase ab | Manduca sexta |
12.12 | - |
phosphorylase a | Manduca sexta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycogen + glucose 1-phosphate | - |
Manduca sexta | glycogen + phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 92000, phosphorylase a, ab and b, SDS-PAGE | Manduca sexta |