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Literature summary for 2.4.1.1 extracted from

  • Matheson, N.K.; Richardson, R.H.
    Kinetic properties of two starch phosphorylases from pea seeds (1973), Phytochemistry, 17, 195-200.
No PubMed abstract available
Search for more literature for 2.4.1.1

Inhibitors

Inhibitors Comment Organism Structure
ADPglucose 1.3 mM, 50% inhibition of phosphorylase II, glucan phosphorolysis; strong inhibition of phosphorolysis, isozyme I Pisum sativum
deoxypyridoxine weak inhibition of phosphorylase I, not II Pisum sativum
additional information not inhibited by fructose 6-phosphate Pisum sativum
pyridine 3-aldehyde weak inhibition of phosphorylase I, not II Pisum sativum
pyridine 4-aldehyde inhibition of phosphorylase I, not II Pisum sativum
pyridoxal phosphorylase I, not II Pisum sativum
pyridoxal 5'-phosphate 74 and 76% inhibition of phosphorylase II and I respectively Pisum sativum
pyridoxamine weak inhibition of phosphorylase I, not II Pisum sativum
pyridoxamine 5'-phosphate weak inhibition of phosphorylase I, not II Pisum sativum
UDPglucose 2-3 mM, 50% inhibition of phosphorylase II, glucan phosphorolysis Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data Pisum sativum
4
-
 glucose 1-phosphate phosphorylase I Pisum sativum
5.5
-
 glucose 1-phosphate phosphorylase II Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
 Victory Freezer
-

Purification (Commentary)

Purification (Comment) Organism
phosphorylase I, starch-Sepharose column, DEAE-cellulose, phosphorylase II, DEAE-cellulose, starch-Sepharose Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Pisum sativum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + glucose 1-phosphate potato amylopectin Pisum sativum amylopectin + phosphate
-
 r
[(1->4)-alpha-D-glucosyl]n + phosphate
-
 Pisum sativum [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate requirement Pisum sativum
pyridoxal 5'-phosphate additional pyridoxalphosphate inhibits Pisum sativum