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Literature summary for 2.4.1.1 extracted from
- Properties of alpha-glucan phosphorylase from pea chloroplasts (1983), Phytochemistry, 22, 1891-1898.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADPglucose | 4 mM, 60% inhibition of chloroplast phosphorylase | Pisum sativum |  |
| glucose 1-phosphate | - |
Pisum sativum | |
| additional information | not inhibited by fructose 6-phosphate; not inhibited by fructose, sucrose, dihydroxyacetone phosphate, 6-phosphogluconate, 2-phosphoglycollate, 2-phosphoglycerate, pyruvate | Pisum sativum | |
| phosphate | - |
Pisum sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic data | Pisum sativum | |
| 0.45 | - |
phosphate | phosphorolysis at pH 7.2 | Pisum sativum | |
| 0.96 | - |
glucose 1-phosphate | synthesis at pH 7.2 | Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Pisum sativum | 9507 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
pea, cv. Kelvedon Wonder | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose, BioGel P-150, partial purification | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Pisum sativum | - |
| shoot | - |
Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.007 | - |
- |
Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (dextrin)n-1 + glucose 1-phosphate | - |
Pisum sativum | (dextrin)n + phosphate | - |
r | |
| amylopectin + glucose 1-phosphate | - |
Pisum sativum | amylopectin + phosphate | - |
r | |
| amylose + phosphate | - |
Pisum sativum | amylose + glucose 1-phosphate | - |
? | |
| glycogen + glucose 1-phosphate | - |
Pisum sativum | glycogen + phosphate | - |
r | |
| starch + glucose 1-phosphate | soluble starch | Pisum sativum | starch + phosphate | - |
? | |
| starch + glucose 1-phosphate | native chloroplast grains | Pisum sativum | starch + phosphate | - |
? | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | no activity with pullulan | Pisum sativum | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
below, synthesis | Pisum sativum |
| 7.2 | - |
phosphorolysis, 50% activity at pH 8.0 | Pisum sativum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.36 | - |
phosphate | at pH 7.2 | Pisum sativum | |
| 5.09 | - |
glucose 1-phosphate | at pH 7.2 | Pisum sativum | |
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