Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADPglucose | kinetics | Chlorella vulgaris | |
additional information | not inhibited by maltose, maltotriose, maltotetraose | Chlorella vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data | Chlorella vulgaris | |
additional information | - |
additional information | amylopectin, 0.69 mg/ml and 0.68 mg/ml for phosphorolysis and synthesis, respectively | Chlorella vulgaris | |
0.21 | - |
maltodecaheptaose | phosphorolysis | Chlorella vulgaris | |
0.5 | - |
maltodecaheptaose | synthesis | Chlorella vulgaris | |
0.68 | - |
maltohexaose | phosphorolysis | Chlorella vulgaris | |
0.72 | - |
maltooctaose | phosphorolysis | Chlorella vulgaris | |
0.93 | - |
maltopentaose | phosphorolysis | Chlorella vulgaris | |
1.22 | - |
maltoheptaose | phosphorolysis | Chlorella vulgaris | |
2 | - |
maltooctaose | synthesis | Chlorella vulgaris | |
2 | 8.3 | maltooctaose | maltoheptaose, maltohexaose, maltopentaose, maltotetraose, synthesis | Chlorella vulgaris | |
2.2 | - |
maltoheptaose | synthesis | Chlorella vulgaris | |
2.5 | - |
maltohexaose | synthesis | Chlorella vulgaris | |
3 | - |
maltopentaose | synthesis | Chlorella vulgaris | |
8.3 | - |
maltotetraose | synthesis | Chlorella vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorella vulgaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Chlorella vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(maltodextrin)n-1 + glucose 1-phosphate | phosphorolysis activity ceases when maltodextrins are degraded to maltotetraose | Chlorella vulgaris | (maltodextrin)n + phosphate | - |
r | |
amylopectin + glucose 1-phosphate | best substrate | Chlorella vulgaris | amylopectin + phosphate | - |
r | |
glycogen + glucose 1-phosphate | rabbit liver glycogen | Chlorella vulgaris | glycogen + phosphate | - |
r | |
glycogen + glucose 1-phosphate | glycogen from oyster | Chlorella vulgaris | glycogen + phosphate | - |
r | |
glycogen + glucose 1-phosphate | glycogen from: rabbit liver | Chlorella vulgaris | glycogen + phosphate | - |
r | |
maltodecaheptaose + phosphate | - |
Chlorella vulgaris | maltodecahexaose + alpha-D-glucose 1-phosphate | - |
r | |
maltoheptadecaose + glucose 1-phosphate | - |
Chlorella vulgaris | maltooctadecaose + phosphate | - |
r | |
maltoheptaose + glucose 1-phosphate | - |
Chlorella vulgaris | maltooctaose + phosphate | - |
r | |
maltohexaose + glucose 1-phosphate | - |
Chlorella vulgaris | maltoheptaose + phosphate | - |
r | |
maltooctaose + glucose 1-phosphate | - |
Chlorella vulgaris | maltononaose + phosphate | - |
r | |
maltopentaose + glucose 1-phosphate | - |
Chlorella vulgaris | maltohexaose + phosphate | - |
r | |
maltotetraose + glucose 1-phosphate | best substrate | Chlorella vulgaris | maltopentaose + phosphate | - |
ir | |
[(1->4)-alpha-D-glucosyl]n + phosphate | ineffective primer: glucose | Chlorella vulgaris | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
[(1->4)-alpha-D-glucosyl]n + phosphate | polyglucose primer required | Chlorella vulgaris | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
ratio of phosphorolysis to synthesis increases significantly at higher temperatures | Chlorella vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.1 | 6.8 | synthesis, 2-(N-morpholino)ethanesulfonic acid buffer | Chlorella vulgaris |
7 | 7.5 | phosphorolysis | Chlorella vulgaris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.6 | 7.5 | approx. half-maximal activity at pH 5.6 and pH 7.5, imidazole buffer, phosphorolysis | Chlorella vulgaris |
7 | 8.1 | maximal activity at pH 7, approx. 60% of maximal activity at pH 8.1, glycylglycine buffer, phosphorolysis | Chlorella vulgaris |