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Literature summary for 2.4.1.1 extracted from
- Glycogen phosphorylase in Dictyostelium. Developmental regulation of two forms and their physical and kinetic properties (1987), J. Biol. Chem., 262, 9486-9493.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 5'-AMP | activation | Dictyostelium discoideum |  |
| 5'-AMP | half-maximal activation of isoenzyme b at approx. 0.7 mM, isozyme a is not activated | Dictyostelium discoideum | |
General Stability
| General Stability | Organism |
|---|---|
| freeze-thawing inactivates | Dictyostelium discoideum |
| Streptomycin sulfate or ammonium sulfate precipitation inactivates b-, not a-isozyme | Dictyostelium discoideum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | isozyme a; weak | Dictyostelium discoideum | |
| high salt concentration | - |
Dictyostelium discoideum | |
| KCl | 80 mM, 50% loss of activity, 200 mM, 90% loss of activity | Dictyostelium discoideum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 2.7 mg/ml, glycogen, isozyme a | Dictyostelium discoideum | |
| additional information | - |
additional information | 4.2 mg/ml, glycogen, isozyme b | Dictyostelium discoideum | |
| 1.2 | - |
phosphate | isoenzyme A | Dictyostelium discoideum | |
| 1.5 | - |
phosphate | isoenzyme b, in the presence of 3 mM 5'-AMP | Dictyostelium discoideum | |
| 55 | - |
phosphate | isoenzyme b, in the absence of 5'-AMP | Dictyostelium discoideum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Dictyostelium discoideum | 5737 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [(1->4)-alpha-D-glucosyl]n + phosphate | Dictyostelium discoideum | key step in cellular differentiation of Dictyostelium discoideum | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dictyostelium discoideum | - |
slime mould, strain Ax3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isozymes a, streptomycin sulfate, ammonium sulfate, 50°C, DE52-cellulose, aminobutyl-agarose, Sephacryl S-300, isoenzyme b, DE52-cellulose, 5'-AMP-Sepharose | Dictyostelium discoideum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.3 | - |
isozyme B | Dictyostelium discoideum |
| 4.3 | - |
isozyme A | Dictyostelium discoideum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 7°C, isozyme b, inactivation within 48 h, isozyme a: at least 48 h, in crude extract | Dictyostelium discoideum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycogen + glucose 1-phosphate | rabbit liver glycogen | Dictyostelium discoideum | glycogen + phosphate | - |
? | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | - |
Dictyostelium discoideum | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | key step in cellular differentiation of Dictyostelium discoideum | Dictyostelium discoideum | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | SDS-PAGE | Dictyostelium discoideum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 26 | - |
- |
Dictyostelium discoideum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 18 | 35 | approx. half-maximal activity at 18°C and 35°C | Dictyostelium discoideum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
inactivation above | Dictyostelium discoideum |
| 50 | - |
inactivation within 5 min, isozyme b | Dictyostelium discoideum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
isozyme a, 2-(N-morpholino)ethanesulfonic acid preferred to imidazole buffer | Dictyostelium discoideum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 7.6 | approx. half-maximal activity at pH 5.8 and 7.6, b-isozyme | Dictyostelium discoideum |
| 5.8 | 7.2 | approx. half-maximal activity at pH 5.8 and 7.2, a-isozyme | Dictyostelium discoideum |
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