Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activation, 5 mM | Streptococcus salivarius | |
cysteine | activation | Streptococcus salivarius | |
Mercaptoacetic acid | 1 mM, activation, inhibition at 5 mM | Streptococcus salivarius | |
p-hydroxymercuribenzoate | slight activation, 0.1-1.0 mM | Streptococcus salivarius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | weak | Streptococcus salivarius | |
Co2+ | - |
Streptococcus salivarius | |
Cu2+ | 2 mM, 98% inhibition | Streptococcus salivarius | |
DTNB | 1 mM, weak | Streptococcus salivarius | |
Fe2+ | 2 mM, 60% inhibition in the presence of 1 mM AMP | Streptococcus salivarius | |
Mg2+ | slight | Streptococcus salivarius | |
Mn2+ | - |
Streptococcus salivarius | |
additional information | not inhibited by 2-mercaptoethanol; not inhibited by Sr2+ | Streptococcus salivarius | |
Sn2+ | - |
Streptococcus salivarius | |
Zn2+ | 2 mM, 63% inhibition in the presence of 1 mM AMP | Streptococcus salivarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 20 mg/ml, oyster glycogen, phosphorolysis, 25 mg/ml, endogen glycogen, synthesis, 27 mg/ml, corn dextrin, phosphorolysis, 40 mg/ml, corn dextrin, synthesis | Streptococcus salivarius | |
additional information | - |
additional information | 42 mg/ml, oyster glycogen, synthesis | Streptococcus salivarius | |
3.4 | - |
glucose 1-phosphate | glycogen synthesis | Streptococcus salivarius | |
3.4 | - |
Glucose-1-phosphate | glycogen synthesis | Streptococcus salivarius | |
20 | - |
phosphate | phosphorolysis of oyster glycogen | Streptococcus salivarius | |
25 | - |
phosphate | phosphorolysis of corn dextrin | Streptococcus salivarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus salivarius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
protamine sulfate, ammonium sulfate, DEAE-Sephadex, Sephadex G-200 | Streptococcus salivarius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.005 | - |
- |
Streptococcus salivarius |
Storage Stability | Organism |
---|---|
-10°C, 200 mM NaF, 1 month, no loss of activity | Streptococcus salivarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(dextrin)n-1 + glucose 1-phosphate | best substrate | Streptococcus salivarius | (dextrin)n + phosphate | - |
r | |
(dextrin)n-1 + glucose 1-phosphate | corn dextrin | Streptococcus salivarius | (dextrin)n + phosphate | - |
r | |
glycogen + glucose 1-phosphate | about half as effective as dextrin | Streptococcus salivarius | glycogen + phosphate | - |
r | |
glycogen + glucose 1-phosphate | glycogen from oyster | Streptococcus salivarius | glycogen + phosphate | - |
r | |
starch + glucose 1-phosphate | glucosylation at 32% the rate of dextrin glucosylation | Streptococcus salivarius | starch + phosphate | - |
? | |
[(1->4)-alpha-D-glucosyl]n + phosphate | - |
Streptococcus salivarius | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
2 h stable | Streptococcus salivarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Streptococcus salivarius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | 7.2 | approx. half-maximal activity at pH 5.7 and 7.2 | Streptococcus salivarius |