Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
1 * 82000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + glyoxylate | Escherichia coli | - |
(S)-malate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap column chromatography | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
in 20 mM Tris, 300 mM NaCl, 10 mM MgSO4, 1 mM TCEP HCl, 10% (v/v) glycerol buffer, pH 7.9 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + glyoxylate | - |
Escherichia coli | (S)-malate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 82000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
malate synthase G | - |
Escherichia coli |
MSG | - |
Escherichia coli |