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Literature summary for 2.3.3.9 extracted from
- Purification and some properties of malate synthase from the methylotropic yeast Hansenula polymorpha (1989), FEMS Microbiol. Lett., 61, 11-16.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
acetyl-CoA | - |
Ogataea angusta |  |
| 0.06 | - |
glyoxylate | - |
Ogataea angusta | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| peroxisome | matrix | Ogataea angusta | 5777 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Ogataea angusta | |
| Mg2+ | Km: 0.3 mM | Ogataea angusta | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
4 * 62000, SDS-PAGE | Ogataea angusta |
| 250000 | - |
gel filtration | Ogataea angusta |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + acetyl-CoA + H2O | Ogataea angusta | enzyme specifically involved in glyoxalate cycle metabolism | (S)-malate + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ogataea angusta | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ogataea angusta |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Ogataea angusta |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, stable for at least 3 months | Ogataea angusta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + acetyl-CoA + H2O | - |
Ogataea angusta | (S)-malate + CoA | - |
? | |
| glyoxylate + acetyl-CoA + H2O | enzyme specifically involved in glyoxalate cycle metabolism | Ogataea angusta | (S)-malate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 62000, SDS-PAGE | Ogataea angusta |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Ogataea angusta |
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Release 2023.1 (January 2023)
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