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Literature summary for 2.3.3.9 extracted from
- Malate synthase from Corynebacterium glutamicum: sequence analysis of the gene and biochemical characterization of the enzyme (1994), Microbiology, 140, 3099-3108.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli and Corynebacterium glutamicum | Corynebacterium glutamicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Corynebacterium glutamicum |  |
| glycolate | - |
Corynebacterium glutamicum | |
| additional information | acetyl-phosphate; not: fructose 1,6-bisphosphate | Corynebacterium glutamicum | |
| oxalate | - |
Corynebacterium glutamicum | |
| phosphoenolpyruvate | not | Corynebacterium glutamicum | |
| pyruvate | not | Corynebacterium glutamicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
acetyl-CoA | - |
Corynebacterium glutamicum | |
| 0.03 | - |
glyoxylate | - |
Corynebacterium glutamicum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 25% of the activation with Mg2+ | Corynebacterium glutamicum | |
| Mg2+ | absolute requirement for divalent cation, maximal activity with 40 mM Mg2+ | Corynebacterium glutamicum | |
| Mn2+ | 15% of the activation with Mg2+ | Corynebacterium glutamicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
gel filtration | Corynebacterium glutamicum |
| 90000 | - |
1 * 90000, SDS-PAGE | Corynebacterium glutamicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | P42450 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Corynebacterium glutamicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28.6 | - |
- |
Corynebacterium glutamicum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for more than 3 weeks | Corynebacterium glutamicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + acetyl-CoA + H2O | - |
Corynebacterium glutamicum | (S)-malate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 90000, SDS-PAGE | Corynebacterium glutamicum |
| monomer | 1* 82362, calculation from nucleotide sequence | Corynebacterium glutamicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 43 | - |
- |
Corynebacterium glutamicum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.27 | - |
oxalate | - |
Corynebacterium glutamicum | |
| 0.44 | - |
glycolate | - |
Corynebacterium glutamicum | |
| 4.3 | - |
ATP | - |
Corynebacterium glutamicum | |
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