Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces arenae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.25 | - |
glyoxylate | - |
Streptomyces arenae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61360 | - |
2 * 61360, calculation from nucleotide sequence | Streptomyces arenae |
110000 | - |
gel filtration | Streptomyces arenae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + acetyl-CoA + H2O | Streptomyces arenae | enzyme specifically involved in glyoxalate cycle metabolism | (S)-malate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces arenae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces arenae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Streptomyces arenae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + acetyl-CoA + H2O | - |
Streptomyces arenae | (S)-malate + CoA | - |
? | |
glyoxylate + acetyl-CoA + H2O | enzyme specifically involved in glyoxalate cycle metabolism | Streptomyces arenae | (S)-malate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 61360, calculation from nucleotide sequence | Streptomyces arenae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
41 | - |
1 h, little effect | Streptomyces arenae |
44 | - |
1 h, complete inactivation | Streptomyces arenae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10.5 | pH 7.5: about 55% of maximal activity, pH 10.5: about 25% of maximal activity, enzyme form MSL and MSH | Streptomyces arenae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
inactive at pH 7.0 and below | Streptomyces arenae |