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Literature summary for 2.3.3.9 extracted from
- Large-scale purification and some properties of malate synthase from bakers yeast (1981), Eur. J. Biochem., 114, 255-262.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glycolate | - |
Saccharomyces cerevisiae |  |
| oxalate | - |
Saccharomyces cerevisiae | |
| oxaloacetate | - |
Saccharomyces cerevisiae | |
| pyruvate | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.083 | - |
acetyl-CoA | - |
Saccharomyces cerevisiae | |
| 0.1 | - |
glyoxylate | - |
Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | binding of acetyl-CoA to the synthase is independent of Mg2+ but that of glyoxylate is strictly dependent on the presence of Mg2+ | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
3 * 66000, SDS-PAGE | Saccharomyces cerevisiae |
| 180000 | - |
high speed equilibrium sedimentation | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA | sequencial random mechanism | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae | (S)-malate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 66000, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI 7.5 | Saccharomyces cerevisiae |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
oxalate | - |
Saccharomyces cerevisiae | |
| 0.31 | - |
glycolate | - |
Saccharomyces cerevisiae | |
| 0.54 | - |
pyruvate | - |
Saccharomyces cerevisiae | |
| 1.5 | - |
oxaloacetate | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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