Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 0.01 mM, 10% activation | Pinus densiflora |
General Stability | Organism |
---|---|
trypsin, treatment for 10 min, about 50% loss of activity of enzyme form MSH, 92% loss of activity of enzyme form MSL | Pinus densiflora |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pinus densiflora | |
EDTA | 1.0 mM, 30% inhibition | Pinus densiflora | |
oxalate | competiteive with glyoxylate | Pinus densiflora | |
phosphoenolpyruvate | - |
Pinus densiflora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | - |
acetyl-CoA | - |
Pinus densiflora | |
0.076 | - |
glyoxylate | - |
Pinus densiflora | |
0.11 | - |
acetyl-CoA | - |
Pinus densiflora | |
0.14 | - |
glyoxylate | - |
Pinus densiflora |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pinus densiflora | |
Mg2+ | optimal concentration 10 mM MgCl2 | Pinus densiflora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
by incubation with 5 mM ATP the high molecular weight enzyme form MSH is converted to the low molecular weight enzyme form MSL | Pinus densiflora |
62000 | - |
x * 62000, SDS-PAGE, enzyme forms MSL and MSH | Pinus densiflora |
65000 | - |
gel filtration, enzyme form MSL | Pinus densiflora |
630000 | - |
gel filtration | Pinus densiflora |
630000 | - |
enzyme form MSH | Pinus densiflora |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pinus densiflora | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pinus densiflora |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:alkane-grown cell | - |
Pinus densiflora | - |
pollen | - |
Pinus densiflora | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36.9 | - |
- |
Pinus densiflora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + acetyl-CoA + H2O | - |
Pinus densiflora | (S)-malate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62000, SDS-PAGE, enzyme forms MSL and MSH | Pinus densiflora |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
heating for 3 min, complete inactivation of enzyme form MSL and 65% loss of activity of enzyme form MSH, slight increase of heat stability in presence of glyoxylate and/or Mg2+ | Pinus densiflora |
30 | - |
pH 7.6, 5 min, enzyme form MSL is stable up to | Pinus densiflora |
40 | - |
pH 7.6, 5 min, about 30% loss of activity of enzyme form MSL | Pinus densiflora |
45 | - |
pH 7.6, 5 min, about 60% loss of activity of enzyme form MSL, enzyme form MSH is stable up to | Pinus densiflora |
50 | - |
pH 7.6, 5 min, complete loss of activity of enzyme form MSL | Pinus densiflora |
55 | - |
pH 7.6, 5 min, complete loss of activity of enzyme form MSH | Pinus densiflora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Pinus densiflora |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | pH 7.0: about 80% of maximal activity, pH 8.7: about 60% of maximal activity, enzyme form MSL and MSH | Pinus densiflora |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
4°C, 12 h, about 60% loss of activity, enzyme form MSH | Pinus densiflora |
6 | - |
4°C, 12 h, about 90% loss of activity, enzyme form MSH | Pinus densiflora |
7 | 8 | 4°C, 12 h, stable, enzyme forms MSL and MSH | Pinus densiflora |
9 | - |
4°C, 12 h, about 50% loss of activity, enzyme form MSL, about 40% loss of activity of enzyme form MSH | Pinus densiflora |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
ATP | enzyme form MSH | Pinus densiflora | |
0.2 | - |
ATP | enzyme form MSL | Pinus densiflora | |
1.2 | - |
oxalate | enzyme form MSH | Pinus densiflora | |
1.5 | - |
oxalate | enzyme form MSL | Pinus densiflora |