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Literature summary for 2.3.3.9 extracted from

  • Fukawa, H.; Ejiri, S.I.; Katsumata, T.
    Purification and some properties of malate synthase from the pollen of Pinus densiflora sieb. et zucc. (1987), Agric. Biol. Chem., 51, 1553-1560.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
EDTA 0.01 mM, 10% activation Pinus densiflora

General Stability

General Stability Organism
trypsin, treatment for 10 min, about 50% loss of activity of enzyme form MSH, 92% loss of activity of enzyme form MSL Pinus densiflora

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Pinus densiflora
EDTA 1.0 mM, 30% inhibition Pinus densiflora
oxalate competiteive with glyoxylate Pinus densiflora
phosphoenolpyruvate
-
Pinus densiflora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.052
-
acetyl-CoA
-
Pinus densiflora
0.076
-
glyoxylate
-
Pinus densiflora
0.11
-
acetyl-CoA
-
Pinus densiflora
0.14
-
glyoxylate
-
Pinus densiflora

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pinus densiflora
Mg2+ optimal concentration 10 mM MgCl2 Pinus densiflora

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
by incubation with 5 mM ATP the high molecular weight enzyme form MSH is converted to the low molecular weight enzyme form MSL Pinus densiflora
62000
-
x * 62000, SDS-PAGE, enzyme forms MSL and MSH Pinus densiflora
65000
-
gel filtration, enzyme form MSL Pinus densiflora
630000
-
gel filtration Pinus densiflora
630000
-
enzyme form MSH Pinus densiflora

Organism

Organism UniProt Comment Textmining
Pinus densiflora
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pinus densiflora

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:alkane-grown cell
-
Pinus densiflora
-
pollen
-
Pinus densiflora
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36.9
-
-
Pinus densiflora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glyoxylate + acetyl-CoA + H2O
-
Pinus densiflora (S)-malate + CoA
-
?

Subunits

Subunits Comment Organism
? x * 62000, SDS-PAGE, enzyme forms MSL and MSH Pinus densiflora

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
heating for 3 min, complete inactivation of enzyme form MSL and 65% loss of activity of enzyme form MSH, slight increase of heat stability in presence of glyoxylate and/or Mg2+ Pinus densiflora
30
-
pH 7.6, 5 min, enzyme form MSL is stable up to Pinus densiflora
40
-
pH 7.6, 5 min, about 30% loss of activity of enzyme form MSL Pinus densiflora
45
-
pH 7.6, 5 min, about 60% loss of activity of enzyme form MSL, enzyme form MSH is stable up to Pinus densiflora
50
-
pH 7.6, 5 min, complete loss of activity of enzyme form MSL Pinus densiflora
55
-
pH 7.6, 5 min, complete loss of activity of enzyme form MSH Pinus densiflora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Pinus densiflora

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5 pH 7.0: about 80% of maximal activity, pH 8.7: about 60% of maximal activity, enzyme form MSL and MSH Pinus densiflora

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
4°C, 12 h, about 60% loss of activity, enzyme form MSH Pinus densiflora
6
-
4°C, 12 h, about 90% loss of activity, enzyme form MSH Pinus densiflora
7 8 4°C, 12 h, stable, enzyme forms MSL and MSH Pinus densiflora
9
-
4°C, 12 h, about 50% loss of activity, enzyme form MSL, about 40% loss of activity of enzyme form MSH Pinus densiflora

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01
-
ATP enzyme form MSH Pinus densiflora
0.2
-
ATP enzyme form MSL Pinus densiflora
1.2
-
oxalate enzyme form MSH Pinus densiflora
1.5
-
oxalate enzyme form MSL Pinus densiflora