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Literature summary for 2.3.3.8 extracted from
- Purification and characterization of ATP:citrate lyase from Hydrogenobacter thermophilus TK-6 (1989), J. Bacteriol., 171, 1788-1792.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Hydrogenobacter thermophilus |  |
| dATP | - |
Hydrogenobacter thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0408 | - |
CoA | - |
Hydrogenobacter thermophilus | |
| 0.65 | - |
ATP | - |
Hydrogenobacter thermophilus | |
| 6.25 | - |
citrate | - |
Hydrogenobacter thermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mn2+ or Mg2+ required | Hydrogenobacter thermophilus | |
| Mg2+ | Km: 8 mM MgCl2 | Hydrogenobacter thermophilus | |
| Mn2+ | Mn2+ or Mg2+ required | Hydrogenobacter thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
6 * 43000, SDS-PAGE | Hydrogenobacter thermophilus |
| 260000 | - |
gel filtration | Hydrogenobacter thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hydrogenobacter thermophilus | - |
- |
- |
| Hydrogenobacter thermophilus TK-6 / IAM 12695 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hydrogenobacter thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26.7 | - |
- |
Hydrogenobacter thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphate + acetyl-CoA + oxaloacetate | - |
Hydrogenobacter thermophilus | ATP + citrate + CoA | - |
r | |
| ADP + phosphate + acetyl-CoA + oxaloacetate | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | ATP + citrate + CoA | - |
r | |
| ATP + citrate + CoA | the synthase activity is less than 0.5% of the ATP:citrate lyase activity | Hydrogenobacter thermophilus | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
r | |
| ATP + citrate + CoA | the synthase activity is less than 0.5% of the ATP:citrate lyase activity | Hydrogenobacter thermophilus TK-6 / IAM 12695 | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
r | |
| dATP + citrate + CoA | - |
Hydrogenobacter thermophilus | dADP + phosphate + acetyl-CoA + oxaloacetate | - |
? | |
| dATP + citrate + CoA | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | dADP + phosphate + acetyl-CoA + oxaloacetate | - |
? | |
| GTP + citrate + CoA | - |
Hydrogenobacter thermophilus | GDP + phosphate + acetyl-CoA + oxaloacetate | - |
? | |
| GTP + citrate + CoA | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | GDP + phosphate + acetyl-CoA + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 43000, SDS-PAGE | Hydrogenobacter thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Hydrogenobacter thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
stable up to | Hydrogenobacter thermophilus |
| 95 | - |
30 min, more than 90% loss of activity in absence of citrate, less than 10% loss of activity in presence of citrate | Hydrogenobacter thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | 6.9 | - |
Hydrogenobacter thermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | stable | Hydrogenobacter thermophilus |
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