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Literature summary for 2.3.3.8 extracted from
- The reductive tricarboxylic acid cycle of carbon dioxide assimilation: initial studies and purification of ATP-citrate lyase from the green sulfur bacterium Chlorobium tepidum (1997), J. Bacteriol., 179, 4859-4867.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
CoA | - |
Chlorobaculum tepidum |  |
| 0.21 | - |
citrate | - |
Chlorobaculum tepidum | |
| 0.31 | - |
ATP | - |
Chlorobaculum tepidum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Km: 4 mM | Chlorobaculum tepidum | |
| Mn2+ | 85% of the activation with Mg2+ | Chlorobaculum tepidum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 135000 | - |
4 * 135000, SDS-PAGE | Chlorobaculum tepidum |
| 550000 | - |
gel filtration | Chlorobaculum tepidum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + citrate + CoA | Chlorobaculum tepidum | key enzyme of CO2 fixation by reductive tricarboxylic acid | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlorobaculum tepidum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | phosphoprotein | Chlorobaculum tepidum |
| side-chain modification | phosphorylation occurs on a His residue at the active site | Chlorobaculum tepidum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Chlorobaculum tepidum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
- |
Chlorobaculum tepidum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + citrate + CoA | - |
Chlorobaculum tepidum | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
? | |
| ATP + citrate + CoA | key enzyme of CO2 fixation by reductive tricarboxylic acid | Chlorobaculum tepidum | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 135000, SDS-PAGE | Chlorobaculum tepidum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 48 | - |
- |
Chlorobaculum tepidum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 57 | 20°C: about 45% of maximal activity, pH 57°C: about 30% of maximal activity | Chlorobaculum tepidum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
loss of activity after 5 min without addition of citrate, loss of activity after 20 min in presence of citrate | Chlorobaculum tepidum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Chlorobaculum tepidum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.2 | maximal stability | Chlorobaculum tepidum |
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