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Literature summary for 2.3.3.5 extracted from
- Crystal structure of Salmonella typhimurium 2-methylcitrate synthase: Insights on domain movement and substrate specificity (2011), J. Struct. Biol., 174, 58-68.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Salmonella enterica subsp. enterica serovar Typhimurium |  |
| pyruvate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
oxaloacetate | using acetyl-CoA as cosubstrate, in 50 mM HEPES pH 8.0, at 30°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.015 | - |
oxaloacetate | using propanoyl-CoA as cosubstrate, in 50 mM HEPES pH 8.0, at 30°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.045 | - |
propanoyl-CoA | in 50 mM HEPES pH 8.0, at 30°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.265 | - |
acetyl-CoA | in 50 mM HEPES pH 8.0, at 30°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | Q56063 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + H2O + oxaloacetate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| butyryl-CoA + H2O + oxaloacetate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| propanoyl-CoA + H2O + oxaloacetate | propanoyl-CoA is the most efficient substrate | Salmonella enterica subsp. enterica serovar Typhimurium | 2-methylcitrate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| decamer | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2-MCS | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| PrpC | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 52 | 60 | the enzyme has a melting temperature of 52°C. Tm increases to 60°C when the enzyme is preincubated with 0.1 mM oxaloacetate | Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.87 | - |
acetyl-CoA | in 50 mM HEPES pH 8.0, at 30°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 8.25 | - |
propanoyl-CoA | in 50 mM HEPES pH 8.0, at 30°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.05 | - |
acetyl-CoA | in 50 mM HEPES pH 8.0, at 30°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 183 | - |
propanoyl-CoA | in 50 mM HEPES pH 8.0, at 30°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
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