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Literature summary for 2.3.3.5 extracted from

  • Domin, N.; Wilson, D.; Brock, M.
    Methylcitrate cycle activation during adaptation of Fusarium solani and Fusarium verticillioides to propionyl-CoA-generating carbon sources (2009), Microbiology, 155, 3903-3912.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pJET1.2 for sequencing, into a modified pET43.1a vector for expression in Escherichia coli BL21DE3 Rosetta 2 cells Fusarium solani
into the vector pJET1.2 for sequencing, into a modified pET43.1a vector for expression in Escherichia coli BL21DE3 Rosetta 2 cells Fusarium verticillioides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00126
-
acetyl-CoA
-
Fusarium solani
0.00188
-
propionyl-CoA
-
Fusarium verticillioides
0.00194
-
propionyl-CoA
-
Fusarium solani
0.00258
-
oxaloacetate propionyl-CoA Fusarium solani
0.00271
-
acetyl-CoA
-
Fusarium verticillioides
0.00281
-
oxaloacetate propionyl-CoA Fusarium verticillioides
0.00385
-
oxaloacetate acetyl-CoA Fusarium solani
0.00964
-
oxaloacetate acetyl-CoA Fusarium verticillioides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
determined by SDS-PAGE Fusarium solani
49000
-
determined by SDS-PAGE Fusarium verticillioides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
propionyl-CoA + H2O + oxaloacetate Fusarium solani
-
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?
propionyl-CoA + H2O + oxaloacetate Fusarium verticillioides
-
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Fusarium solani
-
-
-
Fusarium verticillioides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
on a gravity-flow Ni-NTA agarose column Fusarium solani
on a gravity-flow Ni-NTA agarose column Fusarium verticillioides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.31
-
propionyl-CoA Fusarium verticillioides
19.05
-
acetyl-CoA Fusarium solani
19.55
-
propionyl-CoA Fusarium solani
36.6
-
acetyl-CoA Fusarium verticillioides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate
-
Fusarium verticillioides citrate + CoA
-
?
acetyl-CoA + H2O + oxaloacetate
-
Fusarium solani ? + CoA
-
?
propionyl-CoA + H2O + oxaloacetate
-
Fusarium solani (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?
propionyl-CoA + H2O + oxaloacetate
-
Fusarium verticillioides (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
?

Synonyms

Synonyms Comment Organism
Methylcitrate synthase
-
Fusarium solani
Methylcitrate synthase
-
Fusarium verticillioides

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
49 54 maximum activity Fusarium solani
49 54 maximum activity Fusarium verticillioides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.5
-
propionyl-CoA
-
Fusarium verticillioides
15.5
-
acetyl-CoA
-
Fusarium solani
15.9
-
propionyl-CoA
-
Fusarium solani
29.9
-
acetyl-CoA
-
Fusarium verticillioides

pH Range

pH Minimum pH Maximum Comment Organism
8 9 maximum activity Fusarium solani
8 9 maximum activity Fusarium verticillioides

Cofactor

Cofactor Comment Organism Structure
CoA
-
Fusarium solani
CoA
-
Fusarium verticillioides

General Information

General Information Comment Organism
physiological function propionyl-CoA detoxification Fusarium solani
physiological function propionyl-CoA detoxification Fusarium verticillioides