Application | Comment | Organism |
---|---|---|
medicine | invasive aspergillosis is a life-threatening disease mainly caused by the fungus Aspergillus fumigatus, blocking of methylcitrate synthase abrogates fungal growth and provides a suitable target for new antifungals | Aspergillus fumigatus |
Cloned (Comment) | Organism |
---|---|
into the pCR2.1 and pUChph1 vectors | Aspergillus fumigatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
propionyl-CoA + H2O + oxaloacetate | Aspergillus fumigatus | - |
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
wild-type strain CBS 144.89, methylcitrate synthase mutant DELTAmcsA, complemented methylcitrate synthase mutant 11/12 B1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.003 | - |
wild-type strain, carbon source glucose | Aspergillus fumigatus |
0.004 | - |
complemented methylcitrate synthase mutant 11/12 B1, carbon source glucose | Aspergillus fumigatus |
0.011 | - |
wild-type strain, carbon source olive oil | Aspergillus fumigatus |
0.029 | - |
complemented methylcitrate synthase mutant 11/12 B1, carbon source olive oil | Aspergillus fumigatus |
0.085 | - |
wild-type strain, carbon source peptone | Aspergillus fumigatus |
0.165 | - |
complemented methylcitrate synthase mutant 11/12 B1, carbon source peptone | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | - |
Aspergillus fumigatus | citrate + CoA | - |
? | |
propionyl-CoA + H2O + oxaloacetate | - |
Aspergillus fumigatus | (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Methylcitrate synthase | - |
Aspergillus fumigatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | - |
Aspergillus fumigatus |
General Information | Comment | Organism |
---|---|---|
metabolism | methylcitrate cycle | Aspergillus fumigatus |