Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 CodonPlus (DE3)-GroEL cells | Syntrophus aciditrophicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | 40% inhibition at 10 mM | Syntrophus aciditrophicus | |
4-hydroxymercuribenzoate | complete inhibition at 0.1 mM | Syntrophus aciditrophicus | |
5,5'-dithiobis-2-nitrobenzoate | 30% inhibition at 10 mM | Syntrophus aciditrophicus | |
ATP | 30% inhibition at 10 mM | Syntrophus aciditrophicus | |
citrate | 40% inhibition at 10 mM | Syntrophus aciditrophicus | |
EDTA | complete inhibition at 0.2 mM | Syntrophus aciditrophicus | |
additional information | not inhibited by 5 mM NADH | Syntrophus aciditrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.085 | - |
oxaloacetate | at pH 8.0 and 22°C | Syntrophus aciditrophicus | |
0.13 | - |
acetyl-CoA | at pH 8.0 and 22°C | Syntrophus aciditrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the enzyme contains stoichiometric amounts of Ca2+ (0.9 Ca/73 kDa) | Syntrophus aciditrophicus | |
Co2+ | Co2+ increases the activity by about 70% compared with that obtained withMn2+ | Syntrophus aciditrophicus | |
Mg2+ | Mg2+ has no effect at all on enzyme activity | Syntrophus aciditrophicus | |
Mn2+ | assays are performed in the presence of 0.2 mM MnCl2 | Syntrophus aciditrophicus | |
Zn2+ | the EDTA-inactivated enzyme regains activity by addition Zn2+ at final concentrations of 0.2 mM | Syntrophus aciditrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
4 * 65000, SDS-PAGE | Syntrophus aciditrophicus |
71800 | - |
4 * 71800, calculated from amino acid sequence | Syntrophus aciditrophicus |
72891 | - |
4 * 72891, electrospray-time of flight mass spectrometry | Syntrophus aciditrophicus |
271000 | - |
gel filtration | Syntrophus aciditrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | Syntrophus aciditrophicus | specific substrates | (R)-citrate + CoA | - |
? | |
acetyl-CoA + H2O + oxaloacetate | Syntrophus aciditrophicus ATCC 700169 | specific substrates | (R)-citrate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Syntrophus aciditrophicus | - |
- |
- |
Syntrophus aciditrophicus ATCC 700169 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Strep-tactin column chromatography and Superdex 200 gel filtration | Syntrophus aciditrophicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:crotonate-grown cell | - |
Syntrophus aciditrophicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.72 | - |
purified enzyme, in the absence of Mn2+, at pH 8.0 and 22°C | Syntrophus aciditrophicus |
1.2 | - |
purified enzyme, in the presence of 0.2 mM Mn2+, at pH 8.0 and 22°C | Syntrophus aciditrophicus |
Storage Stability | Organism |
---|---|
-80°C, the oxically purified enzyme, 20% loss of activity after each cycle of freezing at -80°C and thawing | Syntrophus aciditrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | specific substrates | Syntrophus aciditrophicus | (R)-citrate + CoA | - |
? | |
acetyl-CoA + H2O + oxaloacetate | specific substrates | Syntrophus aciditrophicus ATCC 700169 | (R)-citrate + CoA | - |
? | |
additional information | no activity with propionyl-CoA, 2-oxoglutarate, pyruvate, and 2-oxo-3-methylbutanoate | Syntrophus aciditrophicus | ? | - |
? | |
additional information | no activity with propionyl-CoA, 2-oxoglutarate, pyruvate, and 2-oxo-3-methylbutanoate | Syntrophus aciditrophicus ATCC 700169 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 65000, SDS-PAGE | Syntrophus aciditrophicus |
homotetramer | 4 * 71800, calculated from amino acid sequence | Syntrophus aciditrophicus |
homotetramer | 4 * 72891, electrospray-time of flight mass spectrometry | Syntrophus aciditrophicus |
Synonyms | Comment | Organism |
---|---|---|
Re-citrate synthase | - |
Syntrophus aciditrophicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-80 | - |
the oxically purified enzyme loses 20% of its activity after each cycle of freezing at -80°C and thawing | Syntrophus aciditrophicus |