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Literature summary for 2.3.3.21 extracted from

  • Wu, X.; Eiteman, M.A.
    Synthesis of citramalic acid from glycerol by metabolically engineered Escherichia coli (2017), J. Ind. Microbiol. Biotechnol., 44, 1483-1490 .
    View publication on PubMed
Search for more literature for 2.3.3.21

Application

Application Comment Organism
synthesis synthesis of citramalic acid from glycerol by metabolically engineered Escherichia coli Methanocaldococcus jannaschii

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli MG1655 cells Methanocaldococcus jannaschii
the modified citramalate synthase gene cimA from Methanococcus jannaschii is expressed in Escherichia coli. Gene knockouts and fermentation optimization improve citramalate production from glycerol and also reduce acetate accumulation. Near elimination of acetate formation necessitates deletions in genes for both pathways associated with acetate formation: ackA coding acetate kinase, pta coding phosphotransacetylase, and poxB coding pyruvate oxidase Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + pyruvate + H2O Methanocaldococcus jannaschii
-
 CoA + citramalate
-
 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-
Methanocaldococcus jannaschii Q58787
-
-
Methanocaldococcus jannaschii DSM 2661 Q58787
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetate + pyruvate
-
 Methanocaldococcus jannaschii citramalate
-
 ?
acetate + pyruvate
-
 Methanocaldococcus jannaschii DSM 2661 citramalate
-
 ?
acetyl-CoA + pyruvate + H2O
-
 Methanocaldococcus jannaschii CoA + citramalate
-
 ?

Synonyms

Synonyms Comment Organism
CimA
-
 Methanocaldococcus jannaschii
citramalate synthase
-
 Methanocaldococcus jannaschii