Application | Comment | Organism |
---|---|---|
synthesis | synthesis of citramalic acid from glycerol by metabolically engineered Escherichia coli | Methanocaldococcus jannaschii |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli MG1655 cells | Methanocaldococcus jannaschii |
the modified citramalate synthase gene cimA from Methanococcus jannaschii is expressed in Escherichia coli. Gene knockouts and fermentation optimization improve citramalate production from glycerol and also reduce acetate accumulation. Near elimination of acetate formation necessitates deletions in genes for both pathways associated with acetate formation: ackA coding acetate kinase, pta coding phosphotransacetylase, and poxB coding pyruvate oxidase | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + pyruvate + H2O | Methanocaldococcus jannaschii | - |
CoA + citramalate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Methanocaldococcus jannaschii | Q58787 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q58787 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetate + pyruvate | - |
Methanocaldococcus jannaschii | citramalate | - |
? | |
acetate + pyruvate | - |
Methanocaldococcus jannaschii DSM 2661 | citramalate | - |
? | |
acetyl-CoA + pyruvate + H2O | - |
Methanocaldococcus jannaschii | CoA + citramalate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CimA | - |
Methanocaldococcus jannaschii |
citramalate synthase | - |
Methanocaldococcus jannaschii |