Data extracted from this reference:
Inhibitors
5,5'-dithio-bis-(2-nitrobenzoic acid)
Talaromyces trachyspermus
acetyl-CoA
Talaromyces trachyspermus
CoASSCoA
Talaromyces trachyspermus
iodoacetamide
Talaromyces trachyspermus
lauroyl-CoA
strong substrate inhibition, does not follow Michaelis-Menten kinetics
Talaromyces trachyspermus
N-ethylmaleimide
Talaromyces trachyspermus
NADH
at 5 mM, slight inhibition
Talaromyces trachyspermus
p-chloromercuribenzoate
Talaromyces trachyspermus
palmitoyl-CoA
Talaromyces trachyspermus
KM Value [mM]
0.0001
lauroyl-CoA
Talaromyces trachyspermus
0.03
11-formamidoundecanoyl-CoA
Talaromyces trachyspermus
0.035
oxaloacetate
at 0.021 mM lauroyl-CoA
Talaromyces trachyspermus
Molecular Weight [Da]
45000
2 * 45000, SDS-PAGE
Talaromyces trachyspermus
90000
gel filtration
Talaromyces trachyspermus
Natural Substrates/ Products (Substrates)
lauroyl-CoA + H2O + oxaloacetate
Talaromyces trachyspermus
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
?
lauroyl-CoA + H2O + oxaloacetate
Talaromyces trachyspermus Lehman
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
?
Organism
Talaromyces trachyspermus
Talaromyces trachyspermus Lehman
Purification (Commentary)
Talaromyces trachyspermus
Storage Stability
-18°C, 0.5 M (NH4)2SO4, 20% activity loss, 2 months
Talaromyces trachyspermus
Substrates and Products (Substrate)
11-formamidoundecanoyl-CoA + H2O + oxaloacetate
488155
Talaromyces trachyspermus
(2S,3S)-2-hydroxy-11-formamidotridecanoyl + CoA
?
11-formamidoundecanoyl-CoA + H2O + oxaloacetate
488155
Talaromyces trachyspermus Lehman
(2S,3S)-2-hydroxy-11-formamidotridecanoyl + CoA
?
lauroyl-CoA + H2O + oxaloacetate
488155
Talaromyces trachyspermus
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
?
lauroyl-CoA + H2O + oxaloacetate
488155
Talaromyces trachyspermus Lehman
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
?
Subunits
dimer
2 * 45000, SDS-PAGE
Talaromyces trachyspermus
Inhibitors (protein specific)
5,5'-dithio-bis-(2-nitrobenzoic acid)
Talaromyces trachyspermus
acetyl-CoA
Talaromyces trachyspermus
CoASSCoA
Talaromyces trachyspermus
iodoacetamide
Talaromyces trachyspermus
lauroyl-CoA
strong substrate inhibition, does not follow Michaelis-Menten kinetics
Talaromyces trachyspermus
N-ethylmaleimide
Talaromyces trachyspermus
NADH
at 5 mM, slight inhibition
Talaromyces trachyspermus
p-chloromercuribenzoate
Talaromyces trachyspermus
palmitoyl-CoA
Talaromyces trachyspermus
KM Value [mM] (protein specific)
0.0001
lauroyl-CoA
Talaromyces trachyspermus
0.03
11-formamidoundecanoyl-CoA
Talaromyces trachyspermus
0.035
oxaloacetate
at 0.021 mM lauroyl-CoA
Talaromyces trachyspermus
Molecular Weight [Da] (protein specific)
45000
2 * 45000, SDS-PAGE
Talaromyces trachyspermus
90000
gel filtration
Talaromyces trachyspermus
Natural Substrates/ Products (Substrates) (protein specific)
lauroyl-CoA + H2O + oxaloacetate
Talaromyces trachyspermus
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
?
lauroyl-CoA + H2O + oxaloacetate
Talaromyces trachyspermus Lehman
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
?
Purification (Commentary) (protein specific)
Talaromyces trachyspermus
Storage Stability (protein specific)
-18°C, 0.5 M (NH4)2SO4, 20% activity loss, 2 months
Talaromyces trachyspermus
Substrates and Products (Substrate) (protein specific)
11-formamidoundecanoyl-CoA + H2O + oxaloacetate
488155
Talaromyces trachyspermus
(2S,3S)-2-hydroxy-11-formamidotridecanoyl + CoA
?
11-formamidoundecanoyl-CoA + H2O + oxaloacetate
488155
Talaromyces trachyspermus Lehman
(2S,3S)-2-hydroxy-11-formamidotridecanoyl + CoA
?
lauroyl-CoA + H2O + oxaloacetate
488155
Talaromyces trachyspermus
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
?
lauroyl-CoA + H2O + oxaloacetate
488155
Talaromyces trachyspermus Lehman
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
?
Subunits (protein specific)
dimer
2 * 45000, SDS-PAGE
Talaromyces trachyspermus
Other publictions for EC 2.3.3.2
488155
Mahlen
Properties of 2-decylcitrate s ...
Talaromyces trachyspermus, Talaromyces trachyspermus Lehman
Eur. J. Biochem.
22
104-114
1971
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9
3
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2
2
-
5
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1
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1
4
1
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-
-
-
-
-
-
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-
-
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9
-
3
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-
2
2
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-
1
-
-
-
1
4
1
-
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-
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-
-
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-
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-
488156
Mahlen
A metabolic variation in Penic ...
Talaromyces trachyspermus, Talaromyces trachyspermus Lehman
Acta Chem. Scand.
22
2617-2623
1968
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1
1
1
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3
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1
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1
15
-
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1
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1
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-
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1
-
1
-
1
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1
-
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-
1
15
-
1
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-
1
-
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