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show all sequences of 2.3.3.2

Properties of 2-decylcitrate synthase from Penicillium spiculisporum Lehman

Mahlen, A.; Eur. J. Biochem. 22, 104-114 (1971)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithio-bis-(2-nitrobenzoic acid)
-
Talaromyces trachyspermus
acetyl-CoA
-
Talaromyces trachyspermus
CoASSCoA
-
Talaromyces trachyspermus
iodoacetamide
-
Talaromyces trachyspermus
lauroyl-CoA
strong substrate inhibition, does not follow Michaelis-Menten kinetics
Talaromyces trachyspermus
N-ethylmaleimide
-
Talaromyces trachyspermus
NADH
at 5 mM, slight inhibition
Talaromyces trachyspermus
p-chloromercuribenzoate
-
Talaromyces trachyspermus
palmitoyl-CoA
-
Talaromyces trachyspermus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0001
-
lauroyl-CoA
-
Talaromyces trachyspermus
0.03
-
11-formamidoundecanoyl-CoA
-
Talaromyces trachyspermus
0.035
-
oxaloacetate
at 0.021 mM lauroyl-CoA
Talaromyces trachyspermus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, SDS-PAGE
Talaromyces trachyspermus
90000
-
gel filtration
Talaromyces trachyspermus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
lauroyl-CoA + H2O + oxaloacetate
Talaromyces trachyspermus
-
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
-
-
?
lauroyl-CoA + H2O + oxaloacetate
Talaromyces trachyspermus Lehman
-
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Talaromyces trachyspermus
-
-
-
Talaromyces trachyspermus Lehman
-
-
-
Purification (Commentary)
Commentary
Organism
-
Talaromyces trachyspermus
Storage Stability
Storage Stability
Organism
-18°C, 0.5 M (NH4)2SO4, 20% activity loss, 2 months
Talaromyces trachyspermus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11-formamidoundecanoyl-CoA + H2O + oxaloacetate
-
488155
Talaromyces trachyspermus
(2S,3S)-2-hydroxy-11-formamidotridecanoyl + CoA
-
-
-
?
11-formamidoundecanoyl-CoA + H2O + oxaloacetate
-
488155
Talaromyces trachyspermus Lehman
(2S,3S)-2-hydroxy-11-formamidotridecanoyl + CoA
-
-
-
?
lauroyl-CoA + H2O + oxaloacetate
-
488155
Talaromyces trachyspermus
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
-
-
-
?
lauroyl-CoA + H2O + oxaloacetate
-
488155
Talaromyces trachyspermus Lehman
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 45000, SDS-PAGE
Talaromyces trachyspermus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithio-bis-(2-nitrobenzoic acid)
-
Talaromyces trachyspermus
acetyl-CoA
-
Talaromyces trachyspermus
CoASSCoA
-
Talaromyces trachyspermus
iodoacetamide
-
Talaromyces trachyspermus
lauroyl-CoA
strong substrate inhibition, does not follow Michaelis-Menten kinetics
Talaromyces trachyspermus
N-ethylmaleimide
-
Talaromyces trachyspermus
NADH
at 5 mM, slight inhibition
Talaromyces trachyspermus
p-chloromercuribenzoate
-
Talaromyces trachyspermus
palmitoyl-CoA
-
Talaromyces trachyspermus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0001
-
lauroyl-CoA
-
Talaromyces trachyspermus
0.03
-
11-formamidoundecanoyl-CoA
-
Talaromyces trachyspermus
0.035
-
oxaloacetate
at 0.021 mM lauroyl-CoA
Talaromyces trachyspermus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, SDS-PAGE
Talaromyces trachyspermus
90000
-
gel filtration
Talaromyces trachyspermus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
lauroyl-CoA + H2O + oxaloacetate
Talaromyces trachyspermus
-
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
-
-
?
lauroyl-CoA + H2O + oxaloacetate
Talaromyces trachyspermus Lehman
-
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Talaromyces trachyspermus
Storage Stability (protein specific)
Storage Stability
Organism
-18°C, 0.5 M (NH4)2SO4, 20% activity loss, 2 months
Talaromyces trachyspermus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11-formamidoundecanoyl-CoA + H2O + oxaloacetate
-
488155
Talaromyces trachyspermus
(2S,3S)-2-hydroxy-11-formamidotridecanoyl + CoA
-
-
-
?
11-formamidoundecanoyl-CoA + H2O + oxaloacetate
-
488155
Talaromyces trachyspermus Lehman
(2S,3S)-2-hydroxy-11-formamidotridecanoyl + CoA
-
-
-
?
lauroyl-CoA + H2O + oxaloacetate
-
488155
Talaromyces trachyspermus
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
-
-
-
?
lauroyl-CoA + H2O + oxaloacetate
-
488155
Talaromyces trachyspermus Lehman
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 45000, SDS-PAGE
Talaromyces trachyspermus
Other publictions for EC 2.3.3.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
488155
Mahlen
Properties of 2-decylcitrate s ...
Talaromyces trachyspermus, Talaromyces trachyspermus Lehman
Eur. J. Biochem.
22
104-114
1971
-
-
-
-
-
-
9
3
-
-
2
2
-
4
-
-
1
-
-
-
-
1
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
3
-
-
2
2
-
-
-
1
-
-
-
1
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488156
Mahlen
A metabolic variation in Penic ...
Talaromyces trachyspermus, Talaromyces trachyspermus Lehman
Acta Chem. Scand.
22
2617-2623
1968
-
-
-
-
-
1
1
1
-
-
-
-
-
3
-
-
1
-
-
-
-
1
15
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
1
15
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-