Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 0.05 mM, no residual activity. Activtity can be completely restored by addition of Co2+, or Mn2+ | Streptomyces hygroscopicus | |
iodoacetamide | 1 mM, 18% residual activity | Streptomyces hygroscopicus | |
p-chloromercuribenzoate | 0.1 mM, 2% residual activity | Streptomyces hygroscopicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
acetyl-CoA | pH 7.5, 30°C | Streptomyces hygroscopicus | |
0.13 | - |
oxalacetic acid | pH 7.5, 30°C | Streptomyces hygroscopicus | |
0.39 | - |
3-phosphonopyruvate | pH 7.5, 30°C | Streptomyces hygroscopicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | addition completeley restores inactivation by EDTA | Streptomyces hygroscopicus | |
Mn2+ | addition completeley restores inactivation by EDTA | Streptomyces hygroscopicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
2 * 48000, SDS-PAGE | Streptomyces hygroscopicus |
90000 | 98000 | gel filtration | Streptomyces hygroscopicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces hygroscopicus | Q9LCB4 | - |
- |
Streptomyces hygroscopicus SF-1293 | Q9LCB4 | - |
- |
Purification (Comment) | Organism |
---|---|
from cell-free extract | Streptomyces hygroscopicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + 3-phosphonopyruvate | - |
Streptomyces hygroscopicus | (R)-2-(phosphonomethyl)malate + CoA | - |
? | |
acetyl-CoA + H2O + 3-phosphonopyruvate | - |
Streptomyces hygroscopicus SF-1293 | (R)-2-(phosphonomethyl)malate + CoA | - |
? | |
acetyl-CoA + H2O + oxalacetic acid | - |
Streptomyces hygroscopicus | ? + CoA | - |
? | |
additional information | no substrates: propionyl-CoA or butyryl-CoA, pyruvate, alpha-ketoglutarate, deamino-alpha-ketodemethylphosphinothricin or phosphonopyruvate | Streptomyces hygroscopicus | ? | - |
? | |
additional information | no substrates: propionyl-CoA or butyryl-CoA, pyruvate, alpha-ketoglutarate, deamino-alpha-ketodemethylphosphinothricin or phosphonopyruvate | Streptomyces hygroscopicus SF-1293 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 48000, SDS-PAGE | Streptomyces hygroscopicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Streptomyces hygroscopicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Streptomyces hygroscopicus |