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Literature summary for 2.3.3.18 extracted from

  • Shimotohno, K.; Seto, H.; Ōtake, N.; Imai, S.; Murakami, T.
    Studies on the biosynthesis of bialaphos (SF-1293). 8. Purification and characterization of 2-phosphinomethylmalic acid synthase from Streptomyces hygroscopicus SF-1293 (1988), J. Antibiot., 41, 1057-1065.
    View publication on PubMed
Search for more literature for 2.3.3.18

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 0.1 mM, 2% residual activity Streptomyces hygroscopicus
Ca2+ 1 mM, 54% residual activity Streptomyces hygroscopicus
EDTA 0.05 mM, almost complete inactivation Streptomyces hygroscopicus
Fe2+ 1 mM, 50% residual activity Streptomyces hygroscopicus
iodoacetic acid 1 mM, 18% residual activity Streptomyces hygroscopicus
Mg2+ 1 mM, 44% residual activity Streptomyces hygroscopicus
phosphate buffer not inhibitory at 10 mM, 50% loss of activity in 100 mM phosphate buffer Streptomyces hygroscopicus
Zn2+ 1 mM, 24% residual activity Streptomyces hygroscopicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.052
-
 acetyl-CoA pH 8.0, 30°C Streptomyces hygroscopicus
0.13
-
 oxaloacetic acid pH 8.0, 30°C Streptomyces hygroscopicus
0.385
-
 3-(hydroxyphosphinoyl)pyruvate pH 8.0, 30°C Streptomyces hygroscopicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 2 * 48000, SDS-PAGE Streptomyces hygroscopicus
90000 98000 gel fitlration Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate Streptomyces hygroscopicus
-
 phosphinomethylmalate + CoA
-
 ?
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate Streptomyces hygroscopicus SF-1293
-
 phosphinomethylmalate + CoA
-
 ?
acetyl-CoA + H2O + oxaloacetic acid Streptomyces hygroscopicus
-
 ? + CoA
-
 ?
acetyl-CoA + H2O + oxaloacetic acid Streptomyces hygroscopicus SF-1293
-
 ? + CoA
-
 ?
additional information Streptomyces hygroscopicus no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate ?
-
 ?
additional information Streptomyces hygroscopicus SF-1293 no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus Q9LCB4
-
-
Streptomyces hygroscopicus SF-1293 Q9LCB4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces hygroscopicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
546.8
-
 pH 8.0, 30°C Streptomyces hygroscopicus

Storage Stability

Storage Stability Organism
4°C, lyophilized powder, 5% loss of activity within 3 months Streptomyces hygroscopicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
-
 Streptomyces hygroscopicus phosphinomethylmalate + CoA
-
 ?
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
-
 Streptomyces hygroscopicus SF-1293 phosphinomethylmalate + CoA
-
 ?
acetyl-CoA + H2O + oxaloacetic acid
-
 Streptomyces hygroscopicus ? + CoA
-
 ?
acetyl-CoA + H2O + oxaloacetic acid
-
 Streptomyces hygroscopicus SF-1293 ? + CoA
-
 ?
additional information no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate Streptomyces hygroscopicus ?
-
 ?
additional information no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate Streptomyces hygroscopicus SF-1293 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 48000, SDS-PAGE Streptomyces hygroscopicus

Synonyms

Synonyms Comment Organism
2-phosphinomethylmalic acid synthase
-
 Streptomyces hygroscopicus
pmmS
-
 Streptomyces hygroscopicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Streptomyces hygroscopicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Streptomyces hygroscopicus