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Literature summary for 2.3.3.18 extracted from

  • Shimotohno, K.; Seto, H.; Imai, S.; Murakami, T.
    Purification and characterization of citrate synthase from Streptomyces hygroscopicus SF-1293 and comparison of its properties with those of 2-phosphinomethylmalic acid synthase (1990), Agric. Biol. Chem., 54, 463-470.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Co2+ or Mn2+, required Streptomyces hygroscopicus
Mn2+ or Co2+, required Streptomyces hygroscopicus

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate 1 mM, 18% inhibition Streptomyces hygroscopicus
4-chloromercuribenzoate 0.1 mM, 100% inhibition Streptomyces hygroscopicus
ATP 1 mM, 35% inhibition Streptomyces hygroscopicus
EDTA 0.05 mM, 100% inhibition Streptomyces hygroscopicus
iodoacetamide 1 mM, 82% inhibition Streptomyces hygroscopicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.069
-
acetyl-CoA pH 8.0, 30°C Streptomyces hygroscopicus
0.36
-
3-(hydroxyphosphinoyl)pyruvate pH 8.0, 30°C Streptomyces hygroscopicus

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus Q9LCB4
-
-
Streptomyces hygroscopicus SF-1293 Q9LCB4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
-
Streptomyces hygroscopicus phosphinomethylmalate + CoA
-
?
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
-
Streptomyces hygroscopicus SF-1293 phosphinomethylmalate + CoA
-
?