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Literature summary for 2.3.3.17 extracted from

  • Yin, L.; Chen, C.; Chen, G.; Cao, B.; Lei, J.
    Molecular cloning, expression pattern and genotypic effects on glucoraphanin biosynthetic related genes in Chinese kale (Brassica oleracea var. alboglabra Bailey) (2015), Molecules, 20, 20254-20267.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.3.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Brassica oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
-
 Brassica oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an omega-(methylthio)-2-oxoalkanoate + acetyl-CoA + H2O Brassica oleracea
-
 a 2-[omega-(methylthio)alkyl]malate + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Brassica oleracea A0A0B5GPY2 variant alboglabra
-

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Brassica oleracea
-
flower
-
 Brassica oleracea
-
leaf
-
 Brassica oleracea
-
silique
-
 Brassica oleracea
-
stem
-
 Brassica oleracea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an omega-(methylthio)-2-oxoalkanoate + acetyl-CoA + H2O
-
 Brassica oleracea a 2-[omega-(methylthio)alkyl]malate + CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, calculated from amino acid sequence Brassica oleracea

Synonyms

Synonyms Comment Organism
MAM1
-
 Brassica oleracea
methylthioalkylmalate synthase 1
-
 Brassica oleracea

pI Value

Organism Comment pI Value Maximum pI Value
Brassica oleracea calculated from amino acid sequence
-
 6.9

Expression

Organism Comment Expression
Brassica oleracea the enzyme is expressed throughout leaf development with lower transcript levels during the younger stages. The expression level of MAM1 is high at the vegetative-reproductive transition phase and significantly high in genotypes with high glucoraphanin content up