Literature summary for 2.3.3.16 extracted from

Ge, Y.D.; Jiang, L.L.; Hou, S.L.; Su, F.Z.; Wang, P.; Zhang, G.
Heteroexpression and biochemical characterization of thermostable citrate synthase from the cyanobacteria Anabaena sp. PCC7120 (2020), Protein Expr. Purif., 168, 105565 .

Search for more literature for 2.3.3.16

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Nostoc sp. PCC 7120 = FACHB-418

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	5 mM, 64.8% residual activity	Nostoc sp. PCC 7120 = FACHB-418
AMP	5 mM, 60% residual activity	Nostoc sp. PCC 7120 = FACHB-418
ATP	5 mM, 89% residual activity	Nostoc sp. PCC 7120 = FACHB-418
Cu2+	2 mM, almost complete loss of activity	Nostoc sp. PCC 7120 = FACHB-418
dithiothreitol	2 mM, almost complete loss of activity	Nostoc sp. PCC 7120 = FACHB-418
EDTA	2 mM, 59% residual activity	Nostoc sp. PCC 7120 = FACHB-418
NADH	5 mM, 45% residual activity	Nostoc sp. PCC 7120 = FACHB-418
Ni2+	2 mM, 74% residual activity	Nostoc sp. PCC 7120 = FACHB-418
Zn2+	2 mM, 16.8% residual activity	Nostoc sp. PCC 7120 = FACHB-418

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.018	-	acetyl-CoA	pH 8.0, 25°C	Nostoc sp. PCC 7120 = FACHB-418
0.138	-	oxaloacetate	pH 8.0, 25°C	Nostoc sp. PCC 7120 = FACHB-418

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	2 mM, 111% of initial activity	Nostoc sp. PCC 7120 = FACHB-418

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
88533	-	MALDI-TOF	Nostoc sp. PCC 7120 = FACHB-418

Organism

Organism	UniProt	Comment	Textmining
Nostoc sp. PCC 7120 = FACHB-418	Q9R6Y4	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + H2O + oxaloacetate	-	Nostoc sp. PCC 7120 = FACHB-418	citrate + CoA	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 44162.7, MALDI-TOF, 2 * 43000, SDS-PAGE	Nostoc sp. PCC 7120 = FACHB-418

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	-	Nostoc sp. PCC 7120 = FACHB-418

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	half-life about 6.5 h	Nostoc sp. PCC 7120 = FACHB-418

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
23	-	acetyl-CoA	pH 8.0, 25°C	Nostoc sp. PCC 7120 = FACHB-418
31.9	-	oxaloacetate	pH 8.0, 25°C	Nostoc sp. PCC 7120 = FACHB-418

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Nostoc sp. PCC 7120 = FACHB-418

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
230	-	oxaloacetate	pH 8.0, 25°C	Nostoc sp. PCC 7120 = FACHB-418
1270	-	acetyl-CoA	pH 8.0, 25°C	Nostoc sp. PCC 7120 = FACHB-418

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Release 2023.1 (January 2023)