Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.3.16 extracted from

  • Ge, Y.; Hou, S.; Jiang, L.; Su, F.; Wang, P.
    Expression and characterization of a thermostable citrate synthase from Microcystis aeruginosa PCC7806 (2019), FEMS Microbiol. Lett., 366, fnz236 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Microcystis aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 2 mM, 50% residual activity Microcystis aeruginosa
Cu2+ 2 mM, 12% residual activity Microcystis aeruginosa
Mg2+ 2 mM, 0.29% residual activity Microcystis aeruginosa
Mn2+ 2 mM, 72% residual activity Microcystis aeruginosa
Ni2+ 2 mM, 71% residual activity Microcystis aeruginosa
Zn2+ 2 mM, 27% residual activity Microcystis aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0116
-
acetyl-CoA pH 8.0, 25°C Microcystis aeruginosa
0.103
-
oxaloacetate pH 8.0, 25°C Microcystis aeruginosa

Organism

Organism UniProt Comment Textmining
Microcystis aeruginosa A8YCJ7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Microcystis aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate
-
Microcystis aeruginosa citrate + CoA
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Microcystis aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
20 min, 11% loss of activity Microcystis aeruginosa
55
-
half-life about 35 min Microcystis aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.1
-
acetyl-CoA pH 8.0, 25°C Microcystis aeruginosa
24.5
-
oxaloacetate pH 8.0, 25°C Microcystis aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
Microcystis aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
238
-
oxaloacetate pH 8.0, 25°C Microcystis aeruginosa
1474
-
acetyl-CoA pH 8.0, 25°C Microcystis aeruginosa