Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli using the cytoplasmic expression vectors, pET3a and pET3d. The inactive enzyme is reactivated following overnight incubation in 2 M KCl | Haloferax volcanii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | at 0.2 M KCl, approximately 60% of its maximal activity | Haloferax volcanii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
oxaloacetate | 30°C, pH 8.0, native enzyme | Haloferax volcanii | |
0.027 | - |
oxaloacetate | 30°C, pH 8.0, recombinant enzyme | Haloferax volcanii | |
123 | - |
acetyl-CoA | 30°C, pH 8.0, recombinant enzyme | Haloferax volcanii | |
125 | - |
acetyl-CoA | 30°C, pH 8.0, native enzyme | Haloferax volcanii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE | Haloferax volcanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax volcanii | D4GS06 | - |
- |
Haloferax volcanii DSM 3757 | D4GS06 | - |
- |
Purification (Comment) | Organism |
---|---|
recovered using dye-affinity chromatography in the presence of salt | Haloferax volcanii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40 | - |
37°C, pH 8.0 | Haloferax volcanii |
Storage Stability | Organism |
---|---|
room temperature, at 0.2 M KCl, the enzyme is stable at for at least 48 h | Haloferax volcanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | - |
Haloferax volcanii | citrate + CoA | - |
? | |
acetyl-CoA + H2O + oxaloacetate | - |
Haloferax volcanii DSM 3757 | citrate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Haloferax volcanii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Haloferax volcanii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Haloferax volcanii |