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Literature summary for 2.3.3.16 extracted from

  • Karlsson, E.N.; Abou-Hachem, M.; Holst, O.; Danson, M.J.; Hough, D.W.
    Rhodothermus marinus: a thermophilic bacterium producing dimeric and hexameric citrate synthase isoenzymes (2002), Extremophiles, 6, 51-56.
    View publication on PubMed
Search for more literature for 2.3.3.16

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 oxaloacetate dimeric enzyme form with propionyl-CoA Rhodothermus marinus
0.003
-
 propionyl-CoA dimeric enzyme form Rhodothermus marinus
0.003
-
 oxaloacetate dimeric enzyme form with acetyl-CoA Rhodothermus marinus
0.006
-
 oxaloacetate hexameric enzyme form Rhodothermus marinus
0.007
-
 acetyl-CoA dimeric enzyme form Rhodothermus marinus
0.012
-
 acetyl-CoA dimeric enzyme form Rhodothermus marinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 N-terminal protein sequence Rhodothermus marinus
42000
-
 2 * 42000, dimeric enzyme form, SDS-PAGE Rhodothermus marinus
45000
-
 6 * 45000, hexameric enzyme form Rhodothermus marinus
100000
-
 gel filtration Rhodothermus marinus
100000
-
 dimeric enzyme form Rhodothermus marinus
280000
-
 gel filtration Rhodothermus marinus
280000
-
 hexameric enzyme form Rhodothermus marinus

Organism

Organism UniProt Comment Textmining
Rhodothermus marinus
-
 2 enzyme forms
-
Rhodothermus marinus
-
 strain DSM 4252
-

Purification (Commentary)

Purification (Comment) Organism
2 enzyme forms Rhodothermus marinus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28
-
 purified dimeric and hexameric enzyme form Rhodothermus marinus
60
-
 dimeric and hexameric enzyme Rhodothermus marinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O
-
 Rhodothermus marinus citrate + CoA
-
 ?
propionyl-CoA + oxaloacetate + H2O only the dimeric enzyme form, no activity with the hexameric enzyme form Rhodothermus marinus 2-methylcitrate + CoA
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 42000, dimeric enzyme form, SDS-PAGE Rhodothermus marinus
hexamer 6 * 45000, hexameric enzyme form Rhodothermus marinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 enzyme assay at Rhodothermus marinus
85
-
 dimeric and hexameric enzyme forms Rhodothermus marinus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 90
-
 Rhodothermus marinus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 hexameric enzyme form, half-life: 18 min, dimeric enzyme form, half-life: 12 min Rhodothermus marinus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12
-
 propionyl-CoA dimeric enzyme form Rhodothermus marinus
21
-
 acetyl-CoA hexameric enzyme form Rhodothermus marinus
22
-
 acetyl-CoA dimeric enzyme form Rhodothermus marinus