Application | Comment | Organism |
---|---|---|
agriculture | - |
Daucus carota |
agriculture | overexpression of Daucus carota enzyme in Arabidopsis thaliana plant mitochondria improves the ability of the plants to grow in phosphorus limited soil due to enhanced citrate excretion from the roots | Arabidopsis thaliana |
agriculture | overexpression of Daucus carota enzyme in Arabidopsis thaliana plant mitochondria improves the ability of the plants to grow in phosphorus limited soil due to enhanced citrate excretion from the roots | Daucus carota |
Cloned (Comment) | Organism |
---|---|
ectopic overexpression in Arabidopsis thaliana strain WS via infection with Agrobacterium tumefaciens, DNA sequence determination | Daucus carota |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of transgenic Arabidopsis thaliana plants ectopically overexpressing the Daucus carota citrate synthase gene via transformation with Agrobacterium tumefaciens, transgenic plants process the enzyme to its mature form, targeting into mitochondria, increased growth and phosphate accumulation | Daucus carota |
additional information | construction of transgenic Arabidopsis thaliana plants via Agrobacterium infection, functional expression and targeting to the mitochondria | Daucus carota |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Daucus carota | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | Arabidopsis thaliana | - |
citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | Daucus carota | - |
citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | Arabidopsis thaliana WS | - |
citrate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana WS | - |
- |
- |
Daucus carota | - |
- |
- |
Daucus carota | O80433 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | wild-type and transgenis plants, the latter expressing the Daucus carota enzyme | Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00023 | 0.00028 | wild-type | Arabidopsis thaliana |
0.00078 | - |
recombinant in transgenic Arabidopsis thaliana plants | Daucus carota |
0.23 | 0.28 | - |
Arabidopsis thaliana |
0.78 | - |
transgenic Arabidopsis thaliana plants overexpressing the Daucus carota citrate synthase | Daucus carota |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | - |
Arabidopsis thaliana | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | - |
Daucus carota | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | - |
Arabidopsis thaliana WS | citrate + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CS | - |
Arabidopsis thaliana |
CS | - |
Daucus carota |