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Literature summary for 2.3.3.16 extracted from

  • Mitchell, C.G.; Anderson, S.C.K.; El-Mansi, E.M.T.
    Purification and characterization of citrate synthase isoenzymes from Pseudomonas aeruginosa (1995), Biochem. J., 309, 507-511.
No PubMed abstract available
Search for more literature for 2.3.3.16

Activating Compound

Activating Compound Comment Organism Structure
AMP isoenzyme CSI allosteric activation, 360% activity at 1mM, isoenzyme CSII not activated Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
ADP 30% inhibition at 1 mM, only CS II Pseudomonas aeruginosa
ATP isoenzyme CSII, 30% activity at 1 mM, isoenzyme CSI not inhibited at 1 mM Pseudomonas aeruginosa
NADH 78% inhibition by 0.1 mM of CS I, 15% inhibition of CS II at 1 mM; isoenzyme CSI 78% at 0.1 mM, allosteric, isoenzyme CSII 15% activity at 1 mM Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 oxaloacetate CS II Pseudomonas aeruginosa
0.002
-
 oxaloacetate isoenzyme CSII Pseudomonas aeruginosa
0.05
-
 acetyl-CoA CS II Pseudomonas aeruginosa
0.05
-
 acetyl-CoA isoenzyme CSII Pseudomonas aeruginosa
0.86
-
 acetyl-CoA CS I Pseudomonas aeruginosa
0.86
-
 acetyl-CoA isoenzyme CSI Pseudomonas aeruginosa
0.96
-
 oxaloacetate CS I Pseudomonas aeruginosa
0.96
-
 oxaloacetate isoenzyme CSI Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid composition Pseudomonas aeruginosa
36500
-
 2 * 36500, CS II, SDS-PAGE Pseudomonas aeruginosa
37500
-
 isoenzyme CSI 6 * 37500, SDS-PAGE Pseudomonas aeruginosa
42000
-
 6 * 42000, CS i, SDS-PAGE Pseudomonas aeruginosa
42000
-
 isoenzyme CSII 2 * 42000, SDS-PAGE Pseudomonas aeruginosa
80300
-
 CS II, gel filtration Pseudomonas aeruginosa
80300
-
 native CSII, gel filtration Pseudomonas aeruginosa
240000
-
 gel filtration, CS I Pseudomonas aeruginosa
240000
-
 native CSI, gel filtration Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 2 enzyme isoforms with different molecular weight, kinetic and regulatory properties, CS I and CS II
-
Pseudomonas aeruginosa
-
 mutant PAC514
-
Pseudomonas aeruginosa 8602
-
 mutant PAC514
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa
both isoenzymes Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.045
-
 crude extract Pseudomonas aeruginosa
0.76
-
 isoenzyme CSI, Matrex Gel Red-A Pseudomonas aeruginosa
1.32
-
 isoenzymes CSI and CSII, DEAE-Sephacel Pseudomonas aeruginosa
4.7
-
 isozymes CS I and CS II Pseudomonas aeruginosa
4.71
-
 isoenzyme CSI, Sephadex G-200 Pseudomonas aeruginosa
11.5
-
 isoenzyme CSII, Matrex Gel Red-A Pseudomonas aeruginosa

Storage Stability

Storage Stability Organism
4°C, 20% glycerol, 0.2mM oxaloacetate, 6 months, isoenzyme CSII stable, isoenzyme CSI 45% loss of activity Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O
-
 Pseudomonas aeruginosa citrate + CoA
-
 ?
acetyl-CoA + oxaloacetate + H2O
-
 Pseudomonas aeruginosa 8602 citrate + CoA
-
 ?
acetyl-CoA + oxaloacetate + H2O
-
 Pseudomonas aeruginosa citrate + CoA + H+
-
 ?
acetyl-CoA + oxaloacetate + H2O
-
 Pseudomonas aeruginosa 8602 citrate + CoA + H+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 36500, CS II, SDS-PAGE Pseudomonas aeruginosa
dimer isoenzyme CSII 2 * 42000, SDS-PAGE Pseudomonas aeruginosa
hexamer 6 * 42000, CS i, SDS-PAGE Pseudomonas aeruginosa
hexamer isoenzyme CSI 6 * 37500, SDS-PAGE Pseudomonas aeruginosa
More CS I expressed from different structural gene than CS II Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 enzyme assay at Pseudomonas aeruginosa