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Literature summary for 2.3.3.16 extracted from
- Poly-3-hydroxybutyrate production by washed cells of Alcaligenes eutrophus, purification, characterization and potential regulatory role of citrate synthase (1997), Arch. Microbiol., 168, 486-492.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | involved in poly 3-hydroxybutyrate formation | Cupriavidus necator |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.094 | - |
acetyl-CoA | - |
Cupriavidus necator |  |
| 0.12 | - |
oxaloacetate | - |
Cupriavidus necator | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
6 * 47000, SDS-PAGE, or homopentamer | Cupriavidus necator |
| 227000 | - |
gel filtration | Cupriavidus necator |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | Cupriavidus necator | involved in poly 3-hydroxybutyrate synthesis | citrate + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator | - |
basonym Alcaligenes eutrophus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cupriavidus necator |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 64 | - |
purified enzyme | Cupriavidus necator |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | - |
Cupriavidus necator | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | involved in poly 3-hydroxybutyrate synthesis | Cupriavidus necator | citrate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 47000, SDS-PAGE, or homopentamer | Cupriavidus necator |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 34 | - |
enzyme assay at | Cupriavidus necator |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
enzyme assay at | Cupriavidus necator |
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Release 2023.1 (January 2023)
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