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Literature summary for 2.3.3.16 extracted from

  • Russell, R.J.M.; Ferguson, J.M.C.; Hough, D.W.; Danson, M.J.; Taylor, G.L.
    The crystal structure of citrate synthase from the hyperthermophilic archaeon Pyrococcus furiosus at 1.9 Angstroem resolution (1997), Biochemistry, 36, 9983-9994.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, room temperature, 2 mg/ml protein concentration, precipitation by 0.1 M sodium citrate and 0.1 M ammonium phosphate, 20 mM Tris-HCl, pH 8.0, 25 mM KCl, x-ray structure analysis, structural basis of high thermostability Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
secondary and tertiary structure Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q53554
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from E. coli Pyrococcus furiosus

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + H2O + oxaloacetate = citrate + CoA active site Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O
-
Pyrococcus furiosus citrate + CoA
-
?

Subunits

Subunits Comment Organism
More subunit organisation from crystal structure, dimer Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
47
-
half-life 4.7 min Pyrococcus furiosus
70
-
half-life 56.8 min Pyrococcus furiosus
100
-
half-life: 17 min, structural basis Pyrococcus furiosus